CAREER: Syntheses of Hyaluronan Oligosaccharides as Biological Probes

在有机和大分子化学项目的CAREER奖的支持下，托莱多大学化学系的黄雪飞教授将开发基于反应性独立的一锅法策略的新方法，以组装合成的无污染的透明质酸寡糖（sHA）和具有明确结构的sHA类似物库，这将是在分子水平上表征它们的生物相互作用的有用工具，并为开发新的治疗干预提供令人兴奋的线索。sHA文库的成功组装将证明反应性无关的迭代一锅法的能力和优势，并且从这些研究中获得的知识将对寡糖文库的制备非常宝贵。黄雪飞教授在有机和大分子化学项目的支持下，将测定获得的sHA文库对Toll样受体（TLR）-4（一种参与先天免疫的重要受体）的刺激活性。海洛烷寡糖在广泛的生物学事件中发挥重要作用，例如肿瘤细胞生长抑制、多药耐药癌细胞的致敏、血管生成和免疫刺激。研究生，博士后研究人员，本科生和高中生将融入研究。拟议项目的更广泛影响不仅是对理科学生和研究人员的教育，而且是对整个社区的教育。 为了让公众了解科学对社会的影响，黄教授计划举办外展活动，如化学开放日和星期六早晨科学。

With the support of this CAREER Award from the Organic and Macromolecular Chemistry Program, Professor Xuefei Huang, of the Department of Chemistry at the University of Toledo, will develop novel methods based on the reactivity-independent, one-pot strategy to assemble a library of synthetic, contaminant-free hyaluronan oligosaccharides (sHA) and sHA analogs with well defined structures, which will be useful tools for characterization of their biological interactions on a molecular level and provide exciting leads for developments of novel therapeutic interventions. The successful assembly of an sHA library will demonstrate the power and advantages of the reactivity-independent, iterative one-pot approach, and the knowledge gained from these studies will be invaluable to the preparation of oligosaccharide libraries in general.Professor Xuefei Huang, with the support of the Organic and Macromolecular Chemistry Program, will assay the sHA library obtained for their stimulatory activities towards toll like receptor (TLR)-4, an important receptor involved in innate immunity. Hyaluronan oligosaccharides play important roles in a wide range of biological events, such as tumor cell growth suppression, sensitization of multidrug resistant cancer cells, angiogenesis, and immuno-stimulation. Graduate students, postdoctoral researchers, undergraduate and high school students will be integrated into the research. Broader impacts of the proposed project are in education of not only science students and researchers but also the whole community. In order to inform the general public about the impacts of science on society, Professor Huang plans to organize outreach activities such as Chemistry Open House and Saturday Morning Science.