CAREER: Syntheses of Hyaluronan Oligosaccharides as Biological Probes

职业：作为生物探针的透明质酸寡糖的合成

• 批准号: 0547504
• 负责人: Xuefei Huang
• 金额: $ 52.5万
• 依托单位: University of Toledo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0547504&HistoricalAwards=false
• 关键词: CAREER; Syntheses; Hyaluronan; Oligosaccharides; Biological

With the support of this CAREER Award from the Organic and Macromolecular Chemistry Program, Professor Xuefei Huang, of the Department of Chemistry at the University of Toledo, will develop novel methods based on the reactivity-independent, one-pot strategy to assemble a library of synthetic, contaminant-free hyaluronan oligosaccharides (sHA) and sHA analogs with well defined structures, which will be useful tools for characterization of their biological interactions on a molecular level and provide exciting leads for developments of novel therapeutic interventions. The successful assembly of an sHA library will demonstrate the power and advantages of the reactivity-independent, iterative one-pot approach, and the knowledge gained from these studies will be invaluable to the preparation of oligosaccharide libraries in general.Professor Xuefei Huang, with the support of the Organic and Macromolecular Chemistry Program, will assay the sHA library obtained for their stimulatory activities towards toll like receptor (TLR)-4, an important receptor involved in innate immunity. Hyaluronan oligosaccharides play important roles in a wide range of biological events, such as tumor cell growth suppression, sensitization of multidrug resistant cancer cells, angiogenesis, and immuno-stimulation. Graduate students, postdoctoral researchers, undergraduate and high school students will be integrated into the research. Broader impacts of the proposed project are in education of not only science students and researchers but also the whole community. In order to inform the general public about the impacts of science on society, Professor Huang plans to organize outreach activities such as Chemistry Open House and Saturday Morning Science.

在有机和大分子化学项目的CAREER奖的支持下，托莱多大学化学系的黄雪飞教授将开发基于不依赖反应性的一锅策略的新方法，组装一个合成的、无污染的透明质酸寡糖（sHA）和具有明确结构的sHA类似物库。这将是在分子水平上表征它们的生物相互作用的有用工具，并为开发新的治疗干预措施提供令人兴奋的线索。sHA库的成功组装将展示与反应性无关的，迭代的一锅方法的力量和优势，并且从这些研究中获得的知识将对一般低聚糖库的制备具有不可估量的价值。黄雪飞教授将在有机和大分子化学项目的支持下，检测获得的sHA文库对toll样受体(TLR)-4的刺激活性，TLR -4是参与先天免疫的重要受体。透明质酸寡糖在肿瘤细胞生长抑制、多药耐药癌细胞致敏、血管生成和免疫刺激等一系列生物事件中发挥着重要作用。研究生、博士后、本科生和高中生将被纳入研究。拟议项目的更广泛影响不仅是对科学学生和研究人员的教育，而且对整个社会的教育。为了让公众了解科学对社会的影响，黄教授计划举办“化学开放日”和“星期六上午科学”等外展活动。