Micro Bubble Tweezers for Individual Cell Manipulation and In Vitro Ultrasound Cell Therapy

用于单个细胞操作和体外超声细胞治疗的微泡镊子

• 批准号: 0601470
• 负责人: Sung Cho
• 金额: --
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0601470&HistoricalAwards=false
• 关键词: Micro; Bubble; Tweezers; Individual; Cell

This proposal focuses on developing a novel non-invasive integrated system for individual cell manipulation and in vitro ultrasound cell therapy, called bubble tweezers where micro bubbles actuated by electrowetting can manipulate individual cells in parallel and promote delivery of bioactive substances into the cells with ultrasound excitations. Intellectual merit: This project will address fundamental issues in electrowetting actuations of microbubbles and their interactions with objects to be manipulated, and will develop the bubble tweezers based on the gained understanding. The proposed bubble tweezers technology will provide a handy, low-cost, high-throughput cell manipulation tool. Harnessing surface tension enables to generate an extremely strong actuation force (~microNewtons). Soft contacts between cells and bubbles lead to minimal damages on the cells. The integration of the bubble tweezers with ultrasound excitations provides a platform for in-vitro ultrasound cell therapy. This research can increase the success rate in therapeutic cloning and other cell-level therapies (gene injection, in-vitro fertilization, and intracytoplasmic sperm injection), making these clinical benefits one-step closer to everyday life. The integrated platform with ultrasound excitations allows performing numerous in-vitro parallel drug/gene testing on different types of cells, leading to expediting drug developments and advancing ultrasound cell therapy. The proposed manipulation technology can also bring a new tool in micromachine assembling.Broader impact: This research will result in tremendous impacts with societal benefits. This research will also make significant impacts on education by (1) establishing micro/nano scientific visual library on the web from the research results; (2) developing micro/nano science coursework with a lab section for graduates as well as undergraduates; (3) having graduate/undergraduate students participate in research especially from underrepresented groups; and (4) donating the visual results from this project to public museums. Furthermore, the completion of the laboratory facilities during this research will improve the engineering infrastructure at the University of Pittsburgh.

该提案的重点是开发一种新型的非侵入性集成系统，用于单个细胞操作和体外超声细胞治疗，称为气泡镊子，其中由电润湿致动的微气泡可以并行操作单个细胞，并促进生物活性物质在超声激励下递送到细胞中。智力优点：该项目将解决微泡电润湿致动及其与待操纵物体相互作用的基本问题，并将根据所获得的理解开发气泡镊子。提出的气泡镊子技术将提供一个方便，低成本，高通量的细胞操作工具。利用表面张力能够产生极强的驱动力（~微牛顿）。细胞和气泡之间的软接触导致对细胞的最小损伤。气泡镊子与超声激励的集成为体外超声细胞治疗提供了平台。这项研究可以提高治疗性克隆和其他细胞水平治疗（基因注射，体外受精和卵胞浆内精子注射）的成功率，使这些临床益处更接近日常生活。具有超声激励的集成平台允许对不同类型的细胞进行多种体外并行药物/基因测试，从而加快药物开发并推进超声细胞治疗。该技术还可以为微机械装配提供一种新的工具。影响更广泛：本研究将产生巨大的社会效益。这项研究也将通过以下方式对教育产生重大影响：（1）根据研究结果在网上建立微/纳米科学可视化图书馆;（2）为研究生和本科生开发微/纳米科学课程，并提供实验室部分;（3）让研究生/本科生参与研究，特别是来自代表性不足的群体;及（4）将本计划的视觉成果捐赠予公共博物馆。此外，在本研究期间完成的实验室设施将改善匹兹堡大学的工程基础设施。