Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real Time

合作研究：实时超灵敏空气传播病原体检测的集成微系统

• 批准号: 0725525
• 负责人: Sung Cho
• 金额: $ 16万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-11-01 至 2011-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0725525&HistoricalAwards=false
• 关键词: Collaborative; Research; Integrated; Microsystem; Ultrasensitive

Collaborative Research: Integrated Microsystem for Ultrasensitive Airborne Pathogen Detection in Real TimeThe objective of this research is to develop an airborne pathogen sensing system, capable of fast and sensitive environmental monitoring, for use in a broad range of infrastructures. The approach is to develop both microfluidic operations for sample collection and preparation and quantum dots-based nanosensors for identification of pathogens according to their ribosomal RNA sequences. The microfluidic unit and the nanosensor unit will be integrated into a system whose performance will be exemplified through analysis of Bacillus spores. The use of the droplet-based microfluidic operations will minimize the requirement of liquid volume and avoid the need for building moving components on the chip for sample processing that both reduces the consumption of biochemical reagents and the complexity in constructing an integrated microfluidic processing system. The highly sensitive rRNA nanosensors will eliminate the need for polymerase chain reaction (PCR) amplification, thereby simplifying assay protocols and system integration. This sensing system will have broader impacts with the societal benefits including (i) immediate detection of bio-warfare pathogens such as anthrax to prevent their possible catastrophe and (ii) surveillance of environments in, for example, transportation infrastructures, healthcare buildings, and workplaces. Besides, this research will also have significant impacts on education by (i) establishing micro/nano scientific visual library on web based on the research outcome; (ii) developing micro/nano science coursework with a lab session; and (iii) having undergraduates especially from the underrepresented groups to participate in the research activities.

合作研究：集成微系统超灵敏的空气传播病原体检测真实的时间本研究的目的是开发一种空气传播病原体传感系统，能够快速和灵敏的环境监测，用于广泛的基础设施。该方法是开发用于样品收集和制备的微流体操作和用于根据核糖体RNA序列识别病原体的基于量子点的纳米传感器。微流体单元和纳米传感器单元将被集成到一个系统中，其性能将通过分析芽孢杆菌孢子来举例说明。基于液滴的微流体操作的使用将最小化液体体积的需求，并且避免在芯片上构建用于样品处理的移动组件的需要，这既减少了生化试剂的消耗又降低了构建集成微流体处理系统的复杂性。高灵敏度的rRNA纳米传感器将消除对聚合酶链反应（PCR）扩增的需要，从而简化了检测方案和系统集成。该传感系统将具有更广泛的社会效益，包括（i）立即检测生物战病原体，如炭疽，以防止其可能的灾难和（ii）监测环境，例如交通基础设施，医疗保健建筑物和工作场所。此外，这项研究也将通过以下方式对教育产生重大影响：（i）根据研究成果在网络上建立微/纳米科学可视化图书馆;（ii）通过实验室课程开发微/纳米科学课程;以及（iii）特别是来自代表性不足群体的本科生参与研究活动。