Monoamine secretion and synaptic modulation during olfactory conditioning in Drosophila

果蝇嗅觉调节过程中的单胺分泌和突触调节

• 批准号: 175437513
• 负责人: Professor Dr. Stephan J. Sigrist
• 金额: --
• 依托单位: Institut für Biologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/175437513?language=en
• 关键词: Monoamine; secretion; synaptic; modulation; during

Biogenic amines play a pivotal role in orchestration and modulation of behaviors, such as aggression, motivation or learning, in vertebrate and invertebrate species. Within the context of associative memory formation the biogenic amines dopamine (DA) and octopamine (OA) - the invertebrate orthologe to noradrenaline - are of extreme importance as they play a rather complex role: On the one hand, DA and OA are directly involved in memory formation as they mediate rewarding or punishing situations to the animal. On the other hand, DA and OA are involved in control of the animal´s motivational status and thus indirectly control whether a given memory gaines control over behavior. These direct and indirect implications of DA/OA signaling on learning & memory challenge functional analyses.Here we propose to use the well established Drosophila odor learning paradigm to dissect the role of discrete DA or OA receptors (DA-R and OA-R, respectively) on memory formation and behavioral control. To that end we will develop genetic tools to (i) analyze the anatomical organization of distinct receptors within the flies brain by high-resolution STED microscopy and (ii) use tissue specific loss-of-function techniques to investigate their role in memory formation and behavioral expression within the well established odor-learning paradigm.Within the FOR we will tightly collaborate with the group of Stevenson by providing highresolution STED microscopy to the cricket system, with the group of Brembs by providing genetic tools for behavioral analyses in fruit flies and with the group of Eisenhardt by providing antibody expertise to generate antibodies against the bee homologue of the flies bruchpilot gene.

生物胺在脊椎动物和无脊椎动物的攻击性、动机或学习等行为的协调和调节中发挥着关键作用。在联想记忆形成的背景下，生物胺多巴胺(DA)和章鱼胺(OA)--与去甲肾上腺素同源的无脊椎动物--是极其重要的，因为它们扮演着相当复杂的角色：一方面，DA和OA直接参与记忆形成，因为它们调节动物的奖赏或惩罚情况。另一方面，DA和OA参与控制动物的S动机状态，从而间接控制给定记忆是否获得对行为的控制。DA/OA信号在学习和记忆中的这些直接和间接意义对功能分析提出了挑战。在这里，我们建议使用成熟的果蝇气味学习范例来剖析离散的DA或OA受体(分别为DA-R和OA-R)在记忆形成和行为控制中的作用。为此，我们将开发基因工具来(I)通过高分辨率STED显微镜分析苍蝇大脑中不同受体的解剖组织，并(Ii)使用组织特异性功能丧失技术来研究它们在记忆形成和成熟的气味学习范例中的行为表达中的作用。在这方面，我们将通过为板球系统提供高分辨率STED显微镜与Stevenson小组密切合作，通过为果蝇的行为分析提供遗传工具与Brembs组密切合作，通过提供抗体专业知识来产生针对果蝇bruchilot基因的蜜蜂同源物的抗体，与Eisenhardt组合作。