Presynaptic plasticity in the control of mushroom body memory formation

突触前可塑性控制蘑菇体记忆形成

• 批准号: 403500501
• 负责人: Professor Dr. Stephan J. Sigrist
• 金额: --
• 依托单位: Institut für Biologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/403500501?language=en
• 关键词: Presynaptic; plasticity; control; mushroom; body

There is still a fundamental gap in our understanding of how dynamic changes of synapse performance (“plasticity”) intersect with circuit operation and, consequently, define behavioral states. The Drosophila mushroom body (MB) offers for an integrated analysis connecting the molecular/cellular with the behavioral level, here aversive olfactory memory formation and consolidation. We in the last funding period analyzed the role of homeostatic presynaptic plasticity (“PreScale”) for memory formation in the MB Kenyon cells (KCs), a plasticity form we previously found responsible to structurally/functionally remodel presynaptic active zones over 10s of minutes. We found that KC-specific post-developmental knockdown of executory synaptic and regulatory proteins driving PreScale did not affect short-term memory, but robustly interfered with the retrieval of mid-term memories. Still, we still lack an understanding of the spatio-temporal action of this plasticity form within the MB circuitry. We now suggest to reduce the cellular and behavioral complexity of our analysis by restricting the KC neuron subsets analyzed and involve simple learning strategies. Moreover, we strive to directly visualize the plasticity processes taken place at KC synapses, and in connection extend our knowledge concerning the molecular/cell-biological machinery orchestrating PreScale. In result, we hope to arrive at a picture of how circuit refinement uses homeostatic plasticity to allow for the successful circuit integration of to be learned information along the consolidation trajectory.

在我们对突触性能的动态变化(“可塑性”)如何与电路操作相交，从而定义行为状态的理解上，仍然存在着根本的差距。果蝇蘑菇体(MB)提供了一种将分子/细胞与行为水平联系起来的综合分析，这里是厌恶嗅觉记忆的形成和巩固。在上一个资助阶段，我们分析了动态平衡突触前可塑性(PreScale)在MB Kenyon细胞(KCs)记忆形成中的作用，我们之前发现这种可塑性形式在10分钟内对突触前活动区的结构/功能进行了重塑。我们发现，驱动PreScale的可执行突触和调节蛋白的KC特异性发育后敲除不影响短期记忆，但强烈干扰中期记忆的提取。尽管如此，我们仍然缺乏对这种可塑性形式在MB回路中的时空作用的理解。我们现在建议通过限制所分析的KC神经元子集来降低我们分析的细胞和行为的复杂性，并涉及简单的学习策略。此外，我们努力直接可视化发生在KC突触的可塑性过程，并在此基础上扩展我们关于协调PreScale的分子/细胞生物机制的知识。因此，我们希望得出一幅图，说明电路精化如何使用稳态可塑性来允许沿着巩固轨迹学习信息的成功电路集成。