Advancement of a Sustainable Microbial Wastewater Treatment Process for the Removal of Phosphorus

许多出水标准接近0.01至0.02 mgP L-1的值，以防止地表水富营养化。增强型生物除磷（EBPR）被广泛使用，其去除导致出水浓度在1-5毫微克/升之间，需要化学处理以满足更严格的标准。EBPR也经常被证明是不稳定和不可靠的。本研究将提出一个新的EBPR代谢模型，该模型提出该过程是由废水处理中不平衡生长条件导致的微生物严格响应驱动的。通过最大限度地扩大严格响应的范围和持续时间，出水浓度可降至0.01至0.02毫微克/升。这项研究将通过四个互不相干的任务进行。在任务1中，我们将通过对实验室规模生物反应器中真实废水和野生微生物群落的时间序列蛋白质组学数据的多元统计分析，确认与所提出的ebpr - strict响应模型相关的关键代谢过程。任务2和任务3将在生物废水处理中建立最佳反应器操作参数，其中本地微生物联合体将完成可忽略不计的生物除磷水平，同时完成所有其他生物处理目标。任务4将为工艺扩大制定设计和操作标准。对微生物严格反应的新认识将导致更可持续的废水处理，减少对化学品的需求，减少资本和运营成本，并防止生物固体处理的长期问题。

0607329LogeMany effluent standards are approaching values of 0.01 to 0.02 mgP L-1 to prevent eutrophication of surface waters. Enhanced biological phosphorus removal (EBPR) is used extensively with removals resulting in effluent concentrations ranging from 1-5 mgP L-1, requiring chemical treatment to meet stricter standards. EBPR also often proves to be unstable and unreliable. This study will advance a new metabolic model for EBPR that proposes the process is driven by the microbial stringent response that results from unbalanced growth conditions within wastewater treatment. By maximizing the extent and duration of the stringent response, effluent concentrations can be reduced to 0.01 to 0.02 mgP L-1. The study will be undertaken through four discrete tasks. Under Task 1 we will confirm the critical metabolic processes associated with the proposed EBPR-Stringent Response model through multivariate statistical analyses of time-series proteomics data derived from laboratory-scale bioreactors operated with real wastewater and wild microbial consortia. Tasks 2 and 3 will establish the optimum reactor operational parameters within biological wastewater treatment wherein the indigenous microbial consortium will accomplish biological phosphorus removal to negligible levels while concurrently accomplishing all other biological treatment objectives. Task 4 will develop design and operational criteria for process scale-up. The new understanding of the microbial stringent response will result in more sustainable wastewater treatment with decreased demand for chemicals, less capital and operations costs, and prevention of long-term problems with biosolids treatment.