DISSERTATION RESEARCH: Local Adaptation and Gene Flow in a Fragmented Host System: Crepidostomum farionis (Digenea) and Oncorhynchus clarki virginalis (Salmonidae) in New Mexico

论文研究：支离破碎的宿主系统中的局部适应和基因流：新墨西哥州的 Crepidostomum farionis（Digenea）和 Oncorhynchus clarki virginalis（Salmonidae）

• 批准号: 0608244
• 负责人: Thomas Turner
• 金额: $ 1.2万
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0608244&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Local; Adaptation; Gene

This project will examine interactions of the parasitic trematode Crepidostomum farionis and the cutthroat trout Oncorhynchus clarki virginalis (Rio Grande cutthroat) in high elevation streams in northern New Mexico. It will specifically test the hypothesis that pathogen infectivity is negatively related to migration between trout populations, as predicted by recently developed theory. Crepidostomum farionis is a generalist that uses multiple fish hosts, including cutthroat and non-native brown trout, Salmo trutta. Genotypes of individual cutthroat will be determined at a gene locus involved in resistance to parasites, microsatellite, and mitochondrial genes. Migration and gene sharing will be estimated. Likewise, gene sharing between populations of the pathogen will be quantified using amplified fragment length polymorphism (AFLP) markers. This is a new approach in studies of pathogen population dynamics.Studies of host movement, host resistance, and pathogen infectivity have broad potential to reveal how wildlife diseases are spread and maintained in natural populations. This project will also offer volunteer opportunities for undergraduate participation in a multi-disciplinary study. Finally, researchers involved with this project will participate in public education activities organized by both state and federal agencies designed to highlight the status and management of native wildlife within the State of New Mexico.

该项目将研究寄生吸虫Crepidostomum farionis和切喉鳟鱼Oncorhynchus clarki virginalis（格兰德河切喉）在北方新墨西哥州高海拔溪流的相互作用。 它将专门测试的假设，病原体的感染性是负相关的鳟鱼种群之间的迁移，最近发展的理论预测。Crepidostomum farionis是一种多面手，使用多种鱼类宿主，包括割喉和非本地褐鳟鱼，萨尔莫trutta。将在涉及抗寄生虫、微卫星和线粒体基因的基因位点确定个体割喉的基因型。 将对迁移和基因共享进行估计。 同样，病原体种群之间的基因共享将使用扩增片段长度多态性（AFLP）标记进行定量。 这是研究病原菌种群动态的一种新方法，对宿主运动、宿主抗性和病原菌感染性的研究对于揭示野生动物疾病在自然种群中的传播和维持具有广泛的潜力。 该项目还将为本科生参与多学科研究提供志愿者机会。 最后，参与该项目的研究人员将参加由州和联邦机构组织的公共教育活动，这些活动旨在突出新墨西哥州本土野生动物的地位和管理。