Dissertation Research: The Genetic Architecture of Glucosinolate Breakdown Specificity

论文研究：芥子油苷分解特异性的遗传结构

• 批准号: 0608516
• 负责人: Daniel Kliebenstein
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0608516&HistoricalAwards=false
• 关键词: Dissertation; Research; Genetic; Architecture; Glucosinolate

Relatively little is known about the relationship between adaptive phenotypes and the evolution of the underlying genes. One ecologically important phenotype that is constantly undergoing natural selection is plant resistance to insects. This study will uncover genes controlling natural plant insecticides, the glucosinolates, in the model plant Arabidopsis thaliana, employing quantitative and reverse genetics, proteomics, and DNA sequence analysis. These data, and the wealth of publicly available resources in the Arabidopsis community, will show how selection has shaped the genetic architecture of this ecologically and agriculturally important herbivory defense system. This represents an important contribution to the study of phenotypic evolution and it will help resolve several long standing debates in the field. In addition to contributions to the fields of evolution, ecology and agriculture, glucosinolates reduce cancer risks when present in the human diet, thus this study has important implications for human nutrition and agriculture. Additionally, this project will provide for training and research experience for graduate and undergraduate researchers, opportunities for collaboration, and the dissemination of these results to the scientific community.

关于适应性表型与潜在基因进化之间的关系知之甚少。一种不断经历自然选择的重要生态表型是植物对昆虫的抗性。这项研究将利用定量和反向遗传学、蛋白质组学和 DNA 序列分析，揭示模式植物拟南芥中控制天然植物杀虫剂芥子油苷的基因。这些数据以及拟南芥群落中丰富的公开资源将显示选择如何塑造这种在生态和农业上重要的草食防御系统的遗传结构。这代表了对表型进化研究的重要贡献，并将有助于解决该领域几个长期存在的争论。除了对进化、生态和农业领域的贡献外，芥子油苷在人类饮食中存在时还可以降低癌症风险，因此这项研究对人类营养和农业具有重要意义。此外，该项目将为研究生和本科生研究人员提供培训和研究经验、合作机会以及向科学界传播这些成果。