Molekular mechanism of horizontal gene transfer in pathogenic epsilon-proteobacteria

致病性ε-变形菌水平基因转移的分子机制

• 批准号: 175974972
• 负责人: Dr. Kerstin Stingl
• 金额: --
• 依托单位: Bundesinstitut für Risikobewertung (BfR)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/175974972?language=en
• 关键词: Molekular; mechanism; horizontal; gene; transfer

DNA uptake from the environment is the first step for acquisition of new genetic material, including antibiotic resistances as well as virulence traits, enabling bacteria to adapt to changing host environments. In this project, the molecular mechanism of DNA uptake in ε-proteobacteria will be addressed in vitro and in vivo. Taking two important human pathogens, the gastric cancer agent, Helicobacter pylori, and the major cause for human diarrhoea, Campylobacter jejuni, the molecular process of DNA transport across outer and inner membrane will be visualized on the single cell level. Gene products previously identified to be involved in natural transformation in these pathogens will be functionally characterized in respect to their specific role in this multi-step process. Since both pathogens are evolutionary close relatives, but implement completely different DNA uptake systems, they serve as model organisms to decipher differences and common principles of Gram-negative bacterial DNA uptake. This knowledge will give more insight into the molecular mechanism of DNA uptake and may lead to the rational development of inhibitors for natural transformation, reducing bacterial host adaptation and spread of resistances.

从环境中摄取DNA是获得新遗传物质的第一步，包括抗生素抗性和毒力性状，使细菌能够适应不断变化的宿主环境。在本项目中，将在体外和体内研究ε-变形菌摄取DNA的分子机制。以两种重要的人类病原体，胃癌剂幽门螺杆菌和人类腹泻的主要原因空肠弯曲杆菌为例，将在单细胞水平上观察DNA穿过外膜和内膜的分子过程。先前确定的基因产物参与这些病原体的自然转化，将在功能上的特点，在这个多步骤的过程中，他们的具体作用。由于这两种病原体是进化上的近亲，但实施完全不同的DNA摄取系统，因此它们可以作为模式生物来破译革兰氏阴性细菌DNA摄取的差异和共同原则。这些知识将使人们更深入地了解DNA摄取的分子机制，并可能导致自然转化抑制剂的合理开发，减少细菌宿主适应和耐药性的传播。

项目成果

Genetic Diversity as Consequence of a Microaerobic and Neutrophilic Lifestyle

• DOI: 10.1371/journal.ppat.1005626
• 发表时间: 2016-05-01
• 期刊: PLOS PATHOGENS
• 影响因子: 6.7
• 作者: Krueger, Nora-Johanna;Knuever, Marie-Theres;Stingl, Kerstin
• 通讯作者: Stingl, Kerstin