Fluorescent Sensors to Image RNA

有了这个新的奖项，有机和高分子化学项目支持匹兹堡大学化学系的Kazunori Koide教授的工作。这项研究将涉及使用荧光传感器对RNA成像的研究。传感器将与抑制荧光的猝灭剂共价连接。当基因表达时，就会产生一种与猝灭剂结合的RNA。这种猝灭剂结合将允许荧光，从而提供了一种在体内确定哪些基因在活细胞中表达的方法。该项目将开发的技术具有实时检测基因活性的潜力，预计将在化学和生物学的界面上产生广泛而深刻的影响。参与这一研究项目的学生将接受合成、机械和分析化学以及分子、结构和细胞生物学方面的培训。这一跨学科计划将培养出受过独特训练的科学家，能够解决现代化学生物学的复杂问题。

With this new award, the Organic and Macromolecular Chemistry Program supports the work of Professor Kazunori Koide, of the Chemistry Department at the University of Pittsburgh. This research will involve investigations of the use of fluorescent sensors to image RNA. The sensors will be covalently linked to a quencher, which inhibits fluorescence. Upon gene expression, an RNA, which binds to the quencher, will be generated. This quencher binding will allow fluorescence, thus providing an in vivo method of determining which genes are being expressed in a living cell. The techniques to be developed in this project, which have the potential of examining gene activity in real time, are expected to have broad and deep impacts at the interface of chemistry and biology. Students involved in this research program will be trained in synthetic, mechanistic and analytical chemistry, as well as molecular, structural, and cell biology. This interdisciplinary program will produce uniquely trained scientists capable of solving the complex problems of modern chemical biology.