Fluorescent Sensors to Image RNA

有了这个新的奖项，有机和大分子化学项目支持匹兹堡大学化学系Kazunori Koide教授的工作。这项研究将涉及使用荧光传感器对RNA成像的调查。传感器将共价连接到抑制荧光的猝灭剂上。基因表达后，将产生与猝灭剂结合的RNA。这种猝灭剂结合将允许荧光，从而提供一种体内方法来确定哪些基因在活细胞中被表达。该项目开发的技术具有实时检测基因活性的潜力，有望在化学和生物学的界面上产生广泛而深刻的影响。参与本研究计划的学生将接受合成、机械和分析化学，以及分子、结构和细胞生物学方面的培训。这个跨学科项目将培养出能够解决现代化学生物学复杂问题的训练有素的科学家。

With this new award, the Organic and Macromolecular Chemistry Program supports the work of Professor Kazunori Koide, of the Chemistry Department at the University of Pittsburgh. This research will involve investigations of the use of fluorescent sensors to image RNA. The sensors will be covalently linked to a quencher, which inhibits fluorescence. Upon gene expression, an RNA, which binds to the quencher, will be generated. This quencher binding will allow fluorescence, thus providing an in vivo method of determining which genes are being expressed in a living cell. The techniques to be developed in this project, which have the potential of examining gene activity in real time, are expected to have broad and deep impacts at the interface of chemistry and biology. Students involved in this research program will be trained in synthetic, mechanistic and analytical chemistry, as well as molecular, structural, and cell biology. This interdisciplinary program will produce uniquely trained scientists capable of solving the complex problems of modern chemical biology.