Development of Highly Sensitive and Selective Fluorogenic Chemdosimeters

高灵敏、选择性荧光化学剂量计的研制

• 批准号: 1506942
• 负责人: Kazunori Koide
• 金额: $ 46.2万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1506942&HistoricalAwards=false
• 关键词: Development; Highly; Sensitive; Selective; Fluorogenic

In this project funded by the Chemical Structure, Dynamic & Mechanism B program and the Chemical Measurement & Imaging program of the Chemistry Division, Professor Kazunori Koide of the Department of Chemistry at the University of Pittsburgh will develop new classes of chemical reagents with useful fluorescence properties. The goal of this research is to exploit the characteristics of hydrogen peroxide and mercury for the development of fluorescence detection methods. The project lies at the interface of organic, analytical, inorganic, and biological chemistry, and is therefore well suited to the education of scientists at all levels. This group is also well-positioned to provide interdisciplinary education and training for undergraduate and graduate students and post-doctoral researchers. Outreach activities involving freshman college students will be part of the project. Specifically, chemosensors that fluoresce when exposed to mercury or hydrogen peroxide will be developed. For mercury detection, the principal investigator proposes to exploit organic reactions catalyzed by mercury. The catalytic systems will amplify fluorescence signals, yielding highly sensitive analytical methods. The catalytic reactions include a sigmatropic rearrangement and a cyclization reaction, both of which will produce a fluorescent product from a nonfluorescent starting material upon exposure to mercury species. These methods will be applied to the analyses of wastewater from coal-fired power plants. Mercury will be quantified below 0.1 ppb levels. For hydrogen peroxide detection and quantification, a [2,3]-sigmatropic rearrangement mediated by hydrogen peroxide, which creates a green fluorescent dye from a nonfluorescent precursor, will be used. The objective is to develop new probes for detecting and quantifying hydrogen peroxide in living human cells. Toward this objective, nonfluorescent rearrangement substrates will be made. When the substrates react with hydrogen peroxide and the rearrangement occurs, a green fluorescent fluorescein derivative will be produced.

匹兹堡大学化学系的Kazunori Koide教授在化学部的化学结构、动力学机制B项目和化学测量成像项目的资助下，将开发具有有用荧光特性的新型化学试剂。 本研究的目的是利用过氧化氢和汞的特性来开发荧光检测方法。 该项目位于有机，分析，无机和生物化学的界面，因此非常适合各级科学家的教育。 该小组也有能力为本科生和研究生以及博士后研究人员提供跨学科的教育和培训。 涉及大一学生的外联活动将是该项目的一部分。 具体来说，将开发当暴露于汞或过氧化氢时发出荧光的化学传感器。对于汞检测，主要研究者建议利用汞催化的有机反应。 催化系统将放大荧光信号，产生高灵敏度的分析方法。 催化反应包括σ转移重排和环化反应，这两种反应在暴露于汞物质时将从非荧光起始材料产生荧光产物。这些方法将应用于燃煤电厂废水的分析。 汞将被量化为低于0.1 ppb水平。 对于过氧化氢检测和定量，将使用由过氧化氢介导的[2，3]-σ迁移重排，其从非荧光前体产生绿色荧光染料。目的是开发新的探针，用于检测和定量活的人类细胞中的过氧化氢。为了达到这个目的，将制备非荧光重排底物。 当底物与过氧化氢反应并发生重排时，将产生绿色荧光的荧光素衍生物。

项目成果

Third-Generation Method for High-Throughput Quantification of Trace Palladium by Color or Fluorescence

• DOI: 10.1021/acs.oprd.9b00472
• 发表时间: 2020-01-01
• 期刊: ORGANIC PROCESS RESEARCH & DEVELOPMENT
• 影响因子: 3.4
• 作者: Lukomski, Lydia;Pohorilets, Ivanna;Koide, Kazunori
• 通讯作者: Koide, Kazunori

Total Synthesis of the Reported Structure of Stresgenin B Enabled by the Diastereoselective Cyanation of an Oxocarbenium

• DOI: 10.1021/acs.orglett.8b03219
• 发表时间: 2018-12-21
• 期刊: ORGANIC LETTERS
• 影响因子: 5.2
• 作者: Chan, Wei Chuen;Koide, Kazunori
• 通讯作者: Koide, Kazunori

Kinetics and Inverse Temperature Dependence of a Tsuji–Trost Reaction in Aqueous Buffer

• DOI: 10.1021/acscatal.9b03011
• 发表时间: 2019-11
• 期刊: ACS Catalysis
• 影响因子: 12.9
• 作者: Ivanna Pohorilets;Matthew P. Tracey;M. LeClaire;Emily Moore;G. Lu;Peng Liu;K. Koide

Catalysis-Based Fluorometric Method for Trace Palladium Detection with Improved Convenience

• DOI: 10.1021/acs.oprd.0c00377
• 发表时间: 2020-11
• 期刊: Organic Process Research & Development
• 影响因子: 3.4
• 作者: Jessica M. Williams;Annelise K. Wanner;K. Koide