Development of a Bioluminescence Ratio Imaging Microscope (BRIM) to Visualize Biosensors for Signal Transduction Events in Living Cells in Real Time

开发生物发光比成像显微镜（BRIM）以实时可视化活细胞中信号转导事件的生物传感器

• 批准号: 0619631
• 负责人: Albrecht von Arnim
• 金额: $ 40.43万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-10-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0619631&HistoricalAwards=false
• 关键词: Development; Bioluminescence; Ratio; Imaging; Microscope

This award is for the development of a Bioluminescence Ratio Imaging Microscope (BRIM). The BRIM will simultaneously record and image single luminescent photons at two different wavelengths in microscopic specimens that have bioluminescent reporters such as luciferases and aequorin photoproteins. BRIM follows the investigators' previous development of the Bioluminescence Resonance Energy Transfer (BRET) technique. However, the BRET luminescence sensor lacks spectrally resolved microscopic imaging with low-light single-photon sensitivity. Genetically encoded bioluminescent reporters form the basis for powerful and sensitive sensors to record gene expression, dynamic protein-protein interactions, and transient changes in intracellular calcium concentration. These bioluminescent reporters will be detected and imaged with the BRIM. This development project addresses the need for minimally invasive biosensors that can record signal transduction events in living cells in real time. The prototype BRIM system will be available to collaborating researchers at other institutions. The award will provide research training for a graduate student and for undergraduate research interns in an interdisciplinary team of biomedical engineers and molecular cell biologists.

该奖项是为了开发生物发光比率成像显微镜（BRIM）。 BRIM将同时记录和图像的两个不同波长的单发光光子在显微镜标本，有生物发光记者，如发光酶和水母发光蛋白。 BRIM遵循研究人员先前开发的生物发光共振能量转移（BRET）技术。 然而，BRET发光传感器缺乏具有低光单光子灵敏度的光谱分辨显微成像。 基因编码的生物发光报告形成了强大而灵敏的传感器的基础，以记录基因表达，动态蛋白质-蛋白质相互作用和细胞内钙浓度的瞬时变化。 这些生物发光报告分子将被检测到，并与BRIM成像。 该开发项目解决了对微创生物传感器的需求，该传感器可以真实的时间记录活细胞中的信号转导事件。BRIM原型系统将提供给其他机构的合作研究人员。该奖项将提供研究培训的研究生和本科生的研究实习生在一个跨学科的团队的生物医学工程师和分子细胞生物学家。