A micro-plankton detector for deployment in the marine environment

用于海洋环境中部署的微型浮游生物探测器

• 批准号: 0622247
• 负责人: Virginia Armbrust
• 金额: --
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0622247&HistoricalAwards=false
• 关键词: micro; plankton; detector; deployment; marine

The PI's request funding to develop a commercial detector for identifying and counting marine plankton that will be integrated into an instrument for extended use at sea. The micro-plankton detector will be based on the standard detector that Cytopeia uses in its flow cytometers and cell sorters. This detector will be supplemented with position sensitive detectors (PSDs) that sense the position of particles that flow through its observation area. Using well-established principles of flow cytometry and confocal microscopy, the detector will be able to discriminate between particles that flow through the system's optical axis and those particles that pass out of focus. The system will process fluorescence signals from in-focus particles only, and will ignore out-of-focus events that cannot be measured accurately. This will result in will a reliable in situ instrument for routine cytometric characterization of phytoplankton populations.

PI申请资助开发一种用于识别和计数海洋浮游生物的商业探测器，该探测器将被集成到一种仪器中，以便在海上长期使用。微型浮游生物探测器将基于Cytopeia在其流式细胞仪和细胞分选仪中使用的标准探测器。该探测器将配备位置敏感探测器（PSD），用于检测流经其观测区域的粒子的位置。使用流式细胞术和共聚焦显微镜的成熟原理，检测器将能够区分流过系统光轴的颗粒和那些散焦的颗粒。该系统将仅处理来自焦点内颗粒的荧光信号，并将忽略无法准确测量的焦点外事件。这将导致将是一个可靠的原位仪器，用于浮游植物种群的常规细胞计数特征。