SGER: An Autonomous Microbial Genosensor for Freshwater Microbial Ecology

SGER：用于淡水微生物生态学的自主微生物基因传感器

• 批准号: 0639044
• 负责人: Katherine McMahon
• 金额: --
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0639044&HistoricalAwards=false
• 关键词: SGER; Autonomous; Microbial; Genosensor; Freshwater

ABSTRACTThis award will address one of the most significant challenges in studying freshwater bacterial communities; having the ability to remotely sample and detect bacteria at high frequencies and with a high degree of specificity. A prototype insitu sensing system will be constructed with sample collection, filtration, cell lysis, RNA extraction/purification/concentration gene amplification, and data transmission capabilities onboard. The system will be designed for multi-day deployment in lakes and equipped with the ability to detect two common classes of freshwater bacteria. The award will have significant broader impacts through undergraduate, graduate, and minority training.

该奖项将解决淡水细菌群落研究中最重要的挑战之一；具有以高频率和高度特异性远程采样和检测细菌的能力。将构建一个具有样本采集、过滤、细胞裂解、RNA提取/纯化/浓缩、基因扩增和数据传输能力的原位传感系统原型。该系统将设计为在湖泊中进行多天部署，并配备有检测两种常见淡水细菌的能力。该奖项将通过本科生、研究生和少数族裔培训产生重大而广泛的影响。