Collaborative Research: MO: Forces Driving Microbial Community Diversity and Composition in Humic Lakes

合作研究：MO：驱动腐殖湖微生物群落多样性和组成的力量

• 批准号: 0702395
• 负责人: Katherine McMahon
• 金额: $ 81.27万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0702395&HistoricalAwards=false
• 关键词: Collaborative; Research; MO; Forces; Driving

Microbes are responsible for most of the carbon and nutrient cycling in freshwater lakes, and influence both local water quality and global carbon budgets. However, it is not currently possible to predict which types of microbes will be found in a particular lake at a particular time, or to predict how a microbial community would respond to environmental change. Microbial biologists need to learn more about how microbes in lakes assemble into communities, the role of drivers such as competition, predation, resource availability, disturbance, and natural selection in shaping these assemblages. This project addresses the following overarching question: What forces organize microbial communities in humic lakes? Microbes and their communities respond to many forces acting at different spatial and temporal scales, and the effect of these forces can be observed at the level of both communities and populations. An interdisciplinary team of microbiologists, ecologists, engineers, and limnologists will use a suite of molecular tools and genomics to investigate (1) Interactions between bacteria and phytoplankton in the surface layers of lakes; (2) Changes in microbial communities resulting from water column disturbances such as thermal stratification and seasonal mixing and (3) The role of evolutionary processes in shaping individual microbial populations within and among lakes. The results of this work will allow us to better explain and ultimately predict the composition and dynamics of microbial communities and populations in freshwater lakes. This project will not only advance our understanding of the microbial ecology of humic lakes, but of microbial ecology and biodiversity in a broader context. The research approach is structured within an ecological framework and reflect urgent questions in the field of microbial biology. To enhance the broader impacts of the work, the investigators will engage in outreach activities coordinated with the following organizations; the NTL-LTER SchoolYard Science program which promotes inquiry-based learning through workshops for high-school teachers; the Center for Biology Education which is developing a virtual professional development resource for middle and high school science and mathematics teachers; the UW-Madison PEOPLE program which organizes summer courses for underrepresented high-school students; the NSF-funded Center for the Integration of Teaching, Research, and Learning which promotes professional development of graduate students, post-docs, and faculty. The research team will continue to leverage these existing programs to show the general public how freshwater microbes are key components of lake ecosystems, impacting water quality and nutrient cycling in profound ways.

微生物负责淡水湖中的大多数碳和营养循环，并影响当地水质和全球碳预算。但是，目前无法预测特定时间在特定湖泊中会发现哪种类型的微生物，或者预测微生物群落如何应对环境变化。微生物生物学家需要进一步了解湖泊中的微生物如何聚集到社区，竞争，捕食，资源可用性，干扰和自然选择等驾驶员在塑造这些组合中的作用。 该项目解决了以下总体问题：什么力量在腐殖湖中组织微生物群落？ 微生物及其社区对许多在不同的空间和时间尺度上作用的力量做出反应，并且可以在社区和人口的水平上观察到这些力量的效果。微生物学家，生态学家，工程师和林木学家组成的跨学科团队将使用一套分子工具和基因组学来研究（1）在湖泊地面层中细菌与浮游植物之间的相互作用； （2）水柱干扰（如热分层和季节性混合）引起的微生物群落的变化以及（3）进化过程在塑造湖泊内外各个微生物种群中的作用。这项工作的结果将使我们能够更好地解释并最终预测淡水湖中微生物群落和人群的组成和动态。该项目不仅将提高我们对腐殖湖微生物生态学的理解，而且还将在更广泛的背景下对微生物生态学和生物多样性的了解。研究方法是在生态框架内结构的，并反映了微生物生物学领域的紧急问题。为了增强作品的更广泛影响，调查人员将与以下组织协调的外展活动； NTL-lter校园科学计划，通过为高中老师的讲习班促进基于询问的学习；生物学教育中心正在为中学科学和数学教师开发虚拟专业发展资源； UW-Madison People计划，组织夏季课程，为代表性不足的高中生组织； NSF资助的教学，研究和学习融合中心，促进了研究生，培训后和教职员工的专业发展。研究团队将继续利用这些现有计划，向公众展示淡水微生物是湖泊生态系统的关键组成部分，从而以深刻的方式影响水质和营养循环。

项目成果

Diversity, distribution, and expression of opsin genes in freshwater lakes

• DOI: 10.1111/mec.16891
• 发表时间: 2023-03-07
• 期刊: MOLECULAR ECOLOGY
• 影响因子: 4.9
• 作者: He, Shaomei;Linz, Alexandra M.;McMahon, Katherine D.
• 通讯作者: McMahon, Katherine D.