Eco-Systems Biology of Polyphosphate Accumulating Consortia

聚磷酸盐积累聚生体的生态系统生物学

• 批准号: 0967646
• 负责人: Katherine McMahon
• 金额: $ 38.84万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0967646&HistoricalAwards=false
• 关键词: Eco; Systems; Biology; Polyphosphate; Accumulating

0967646McMahonThis NSF award by the Biotechnology, Biochemical and Biomass Engineering program supports work to use a systems biology framework to understand the behavior of an engineered ecosystem. The ecosystem is a wastewater treatment process designed to biologically remove phosphorus from sewage in order to protect surface waters from excessive nutrient inputs. The organisms responsible for phosphorus removal (called Accumulibacter) use a unique and complex metabolism that is poorly understood. In this project, we will use novel techniques based in meta-omics to study gene expression at the level of the entire bacterial community in a laboratory scale wastewater treatment system. The integrative and holistic approach provided by systems biology will allow us to understand how different species of Accumulibacter regulate their biochemical pathways to maximize phosphorus removal from water. Finally, we will construct a computational model that can predict how the Accumulibacter metabolism functions under normal and perturbed conditions. This understanding will improve our ability to predict when and why wastewater treatment systems fail, but it will also serve as a powerful case study in the application of systems biology tools to bacterial communities that are engineered using ecological principles.

0967646MCMAHONTHIS NSF颁发的生物技术，生化和生物量工程计划支持使用系统生物学框架来了解工程生态系统的行为。生态系统是一种废水处理过程，旨在从污水中清除磷，以保护地表水免受过多的养分输入。负责去除磷的生物（称为累积杆菌）使用一种独特而复杂的代谢，这是鲜为人知的。在这个项目中，我们将使用基于元词的新技术来研究实验室规模的废水处理系统中整个细菌群落水平上的基因表达。系统生物学提供的综合和整体方法将使我们能够了解不同种类的蓄积种类如何调节其生化途径以最大程度地从水中去除磷。最后，我们将构建一个计算模型，该模型可以预测在正常和扰动条件下如何在正常和扰动条件下起作用。这种理解将提高我们预测废水处理系统何时以及为什么失败的能力，但它也将在应用于使用生态学原理设计的细菌群落中应用系统生物学工具中有力研究。