SGER: The Enhanced Biological Phosphorus Removal Metaproteome

SGER：增强型生物除磷元蛋白质组

• 批准号: 0634304
• 负责人: Katherine McMahon
• 金额: $ 7万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0634304&HistoricalAwards=false
• 关键词: SGER; Enhanced; Biological; Phosphorus; Removal

0634304McMahonThe biochemical mechanisms of enhanced biological phosphorus removal (EBPR) from wastewater are poorly understood because of the lack of pure cultures of the bacteria responsible for the complex metabolism of this important and common treatment process. The lack of pure cultures reflects repeated failures to isolate these microbes. Modern molecular tools now enable the analysis of the structure and function of mixed microbial communities in ways that were previously limited to pure cultures. Until recently, attempts to study the EBPR metaproteome (i.e., the proteome of the mixed microbial community responsible for EBPR) also were limited by lack of availability of its metagenome sequence. The objective of the research is to advance understanding of the microbiology of EBPR by analyzing its metaproteome. The project will use the newly sequenced EBPR metagenome and apply state-of-the-art mass spectrometry, specifically the new method of quantitative 2D-LC-MS/MS, in a first-time application of proteomics to an important wastewater treatment process. The project will demonstrate the usefulness of new proteomics techniques in understanding the behavior of mixed microbial communities responsible for important wastewater treatment processes like EBPR that typically are difficult to manage. A graduate student in Environmental Engineering will be trained as part of the project. The PI will feature this work in an annual visit to a middle school to introduce environmental engineering and science to sixth graders. This project fits the SGER criteria in that it is innovative and "high risk-high reward" and breaks new ground.

0634304MCMAHON，由于缺乏负责这种重要和常见治疗过程的复杂代谢的细菌的纯净培养物，因此对废水的生物学去除（EBPR）的生化机制知之甚少。缺乏纯培养物反映了隔离这些微生物的反复失败。现代分子工具现在可以以以前仅限于纯培养的方式来分析混合微生物群落的结构和功能。直到最近，还尝试研究EBPR元蛋白酶（即负责EBPR的混合微生物群落的蛋白质组）的尝试也受到其元基因组序列的可用性的限制。该研究的目的是通过分析其元蛋白酶来提高对EBPR的微生物学的理解。该项目将使用新测序的EBPR元基因组并应用最先进的质谱法，特别是定量2D-LC-MS/MS的新方法，将蛋白质组学首次应用于重要的废水处理过程。该项目将证明新蛋白质组学技术在理解负责重要废水处理过程（例如EBPR）的混合微生物群落的行为方面的有用性，而EBPR通常难以管理。环境工程研究生将​​作为该项目的一部分进行培训。 PI将在对中学的年度访问中进行这项工作，以向六年级学生介绍环境工程和科学。该项目符合SGER标准，因为它具有创新性和“高风险最高奖励”并打破了新的基础。