SGER: The Enhanced Biological Phosphorus Removal Metaproteome

SGER：增强型生物除磷元蛋白质组

• 批准号: 0634304
• 负责人: Katherine McMahon
• 金额: $ 7万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0634304&HistoricalAwards=false
• 关键词: SGER; Enhanced; Biological; Phosphorus; Removal

0634304McMahonThe biochemical mechanisms of enhanced biological phosphorus removal (EBPR) from wastewater are poorly understood because of the lack of pure cultures of the bacteria responsible for the complex metabolism of this important and common treatment process. The lack of pure cultures reflects repeated failures to isolate these microbes. Modern molecular tools now enable the analysis of the structure and function of mixed microbial communities in ways that were previously limited to pure cultures. Until recently, attempts to study the EBPR metaproteome (i.e., the proteome of the mixed microbial community responsible for EBPR) also were limited by lack of availability of its metagenome sequence. The objective of the research is to advance understanding of the microbiology of EBPR by analyzing its metaproteome. The project will use the newly sequenced EBPR metagenome and apply state-of-the-art mass spectrometry, specifically the new method of quantitative 2D-LC-MS/MS, in a first-time application of proteomics to an important wastewater treatment process. The project will demonstrate the usefulness of new proteomics techniques in understanding the behavior of mixed microbial communities responsible for important wastewater treatment processes like EBPR that typically are difficult to manage. A graduate student in Environmental Engineering will be trained as part of the project. The PI will feature this work in an annual visit to a middle school to introduce environmental engineering and science to sixth graders. This project fits the SGER criteria in that it is innovative and "high risk-high reward" and breaks new ground.

由于缺乏负责这一重要而常见的处理过程的复杂新陈代谢的细菌的纯培养，因此对废水中强化生物除磷(EBPR)的生化机理知之甚少。缺乏纯培养反映了这些微生物一再未能分离出来。现代分子工具现在能够以以前仅限于纯培养的方式分析混合微生物群落的结构和功能。直到最近，对EBPR元蛋白质组(即负责EBPR的混合微生物群落的蛋白质组)的研究也因缺乏其元基因组序列而受到限制。本研究的目的是通过分析EBPR的代谢蛋白质组来促进对其微生物学的了解。该项目将使用新测序的EBPR元基因组，并应用最先进的质谱学，特别是定量2D-LC-MS/MS的新方法，首次将蛋白质组学应用于一个重要的废水处理过程。该项目将展示新的蛋白质组学技术在了解负责EBPR等重要废水处理过程的混合微生物群落行为方面的有效性，这些过程通常很难管理。作为该项目的一部分，将对一名环境工程研究生进行培训。PI将在一所中学的年度访问中展示这项工作，向六年级的学生介绍环境工程和科学。这个项目符合SGER的标准，因为它具有创新性和“高风险-高回报”，并具有开创性。