CRIF:MU Upgrade of Spectroscopy Tools for Biophysics, Surface Chemistry, Single-Molecule Science and Nanotechnology Research and Education

获得化学研究仪器与设备协会颁发的奖项：多用户计划（CRIF:MU），斯坦福大学化学系将升级其用于教学和研究的红外和荧光光谱设备，包括具有空间分辨率的红外显微镜，FTIR单层分析和漫反射附件，并升级带有停止流动模块的荧光仪，用于快速混合能力和时间分辨率测量反应速率至毫秒级。这些仪器将用于各种研究项目，包括使用单分子荧光光谱研究蛋白质构象变化，与集合测量进行比较；广泛的生物系统研究包括绿色荧光蛋白（GFP）变异，振动斯塔克效应（VSE）光谱，以及空间分辨FTIR模型膜表面分析；碳纳米管末端和侧壁加氢化学的空间解析及其在储氢和纳米管传感器中的潜在应用研究研究开发碳的表面功能化化学，包括石墨和碳纳米管，对燃料电池，电池和生物传感器的发展很重要。这些仪器将用于将纳米技术元素引入化学系的本科教学，并计划在实验课程中进行实验。这些仪器将被本科生、研究生和博士后在他们的研究中使用，并将受益于各种外展项目，如RET、REU和SURE，以及斯坦福大学nsf资助的中心，包括CPIMA和NSEC的研究。

With this award from the Chemistry Research Instrumentation and Facilities: Multi User program (CRIF:MU), the Chemistry Department at Stanford University will upgrade their infra red and fluorescence spectrometry equipment for teaching and research with acquisition of an infrared microscope with spatial resolution, FTIR monolayer analysis and diffuse reflectance accessories, and upgrade a fluorimeter with a stopped-flow module for rapid mixing capabilities and time resolution measurement of reaction rates down to the millisecond regime. The instruments will be used in various research projects including the study of protein conformational changes using single molecule fluorescence spectroscopy to make comparisons with ensemble measurements; investigations of a wide range of biological systems include green fluorescent protein (GFP) variants, vibrational Stark effect (VSE) spectroscopy, and the analysis of model membrane surfaces with spatially resolved FTIR; investigations to spatially resolve carbon nanotube hydrogenation chemistry at the ends and sidewalls with potential applications in hydrogen storage and nanotube nansensors; studies to develop surface functionalization chemistry for carbon, including graphite and carbon nanotubes, important to fuel cells, batteries and biosensor developments. The instruments will be used to introduce a nanotechnology element into undergraduate teaching in the chemistry department with experiments planned for a laboratory course. The instruments will be used by undergraduate, graduate students and postdoctoral fellows in their research and will benefit various outreach programs such as RET, REU and SURE, and the research of NSF-funded centers at Stanford including CPIMA and NSEC.