Collaborative Research: Comparative Biogeography of Sulawesi - Phylogenetic and Coalescent Analyses of Diversification in Frogs, Lizards, and Monkeys

苏拉威西岛的比较生物地理学-青蛙、蜥蜴和猴子多样性的系统发育和联合分析生物地理学家长期以来一直对印度尼西亚苏拉威西岛高度特有的生物群感兴趣。苏拉威西在整个历史上都与邻近的大陆隔绝，是由多个起源于西太平洋偏远角落的古岛屿堆积而成的。这项研究将通过应用系统发育和生物地理方法以及最近开发的基于融合的种群遗传学方法来研究苏拉威西动物的物种多样性和生物地理学。分类的重点将是六个脊椎动物分类群，包括特有的苏拉威西辐射猕猴(Macaca)、飞行蜥蜴(Draco)、树皮(Lamproepis)、尖牙蛙(Limnon Ectes)、蟾蜍(Bufo名人)和树蛙(Polypeates)。这项研究的一个新方面是为每个焦点分类单元开发20个未连接的核基因座，通过筛选为每个组构建的基因组文库获得。将使用系统发生学方法对这些基因座进行分析，以评估整个东南亚的广泛生物地理模式和苏拉威西岛的详细多样化模式。他们将使用联合方法进行进一步分析，以调查物种边界的基因流动，并估计苏拉威西物种形成事件的分歧时间。结果将直接适用于印度尼西亚负责保护生物多样性的机构，以针对这个全球保护热点地区保护不足的地方病地区。

Comparative Biogeography of Sulawesi - Phylogenetic and Coalescent Analysis of Diversification in Frogs, Lizards, and MonkeysBiogeographers have long been interested in the highly endemic biota of the Indonesian island of Sulawesi. Sulawesi has been isolated from the adjacent mainland for its entire history, and was formed through accretion of multiple paleo-islands that originated in far-flung corners of the West Pacific. This study will investigate species diversification and biogeography of Sulawesi fauna by applying phylogenetic and biogeographic methodologies, along with recently developed coalescent-based population genetic approaches. The taxonomic focus will be six vertebrate taxa comprising endemic Sulawesi radiations of macaque monkeys (Macaca), flying lizards (Draco), tree skinks (Lamprolepis), fanged frogs (Limnonectes), toads (Bufo celebensis), and tree frogs (Polypedates).A novel aspect of this study is the development of 20 unlinked nuclear loci for each focal taxon obtained by screening genomic libraries constructed for each group. These loci will be analyzed using phylogenetic methods to evaluate broad biogeographic patterns across SE Asia and detailed patterns of diversification on Sulawesi. They will be further analyzed using coalescent methods to investigate gene flow at species boundaries, and to estimate divergence times for speciation events on Sulawesi. Results will be directly applicable to Indonesian agencies tasked with protecting biodiversity to target underprotected areas of endemism in this Global Conservation hotspot.