Collaborative Research: Comparative Biogeography of Sulawesi - Phylogenetic and Coalescent Analyses of Diversification in Frogs, Lizards, and Monkeys

苏拉威西岛的比较生物地理学--青蛙、蜥蜴和猴子多样性的系统发育和融合分析生物地理学家长期以来一直对印度尼西亚苏拉威西岛的高度地方性生物群感兴趣。苏拉威西岛在整个历史上一直与邻近的大陆隔离，是由多个起源于西太平洋偏远角落的古岛屿堆积而成。这项研究将调查物种多样性和物种地理学的苏拉威西动物群应用系统发育和地理学的方法，沿着最近开发的基于聚结的人口遗传学的方法。分类学重点将是六个脊椎动物分类群，包括苏拉威西特有的猕猴（Macaca）、飞蜥（Draco）、树蜥（Lamprolepis）、尖牙蛙（Limnonectes）、蟾蜍（Bufo celebensis）和树蛙（Polypedates）。本研究的一个新方面是为每个焦点分类群开发20个非连锁核基因座，通过筛选为每个群体构建的基因组库获得。这些位点将使用系统发育方法进行分析，以评估广泛的地理模式在东南亚和苏拉威西岛的多样化的详细模式。他们将进一步分析使用合并的方法，调查在物种边界的基因流，并估计在苏拉威西岛物种形成事件的分歧时间。研究结果将直接适用于负责保护生物多样性的印度尼西亚机构，以针对这一全球保护热点地区保护不足的地方性地区。

Comparative Biogeography of Sulawesi - Phylogenetic and Coalescent Analysis of Diversification in Frogs, Lizards, and MonkeysBiogeographers have long been interested in the highly endemic biota of the Indonesian island of Sulawesi. Sulawesi has been isolated from the adjacent mainland for its entire history, and was formed through accretion of multiple paleo-islands that originated in far-flung corners of the West Pacific. This study will investigate species diversification and biogeography of Sulawesi fauna by applying phylogenetic and biogeographic methodologies, along with recently developed coalescent-based population genetic approaches. The taxonomic focus will be six vertebrate taxa comprising endemic Sulawesi radiations of macaque monkeys (Macaca), flying lizards (Draco), tree skinks (Lamprolepis), fanged frogs (Limnonectes), toads (Bufo celebensis), and tree frogs (Polypedates).A novel aspect of this study is the development of 20 unlinked nuclear loci for each focal taxon obtained by screening genomic libraries constructed for each group. These loci will be analyzed using phylogenetic methods to evaluate broad biogeographic patterns across SE Asia and detailed patterns of diversification on Sulawesi. They will be further analyzed using coalescent methods to investigate gene flow at species boundaries, and to estimate divergence times for speciation events on Sulawesi. Results will be directly applicable to Indonesian agencies tasked with protecting biodiversity to target underprotected areas of endemism in this Global Conservation hotspot.