IDBR: Development of High-Speed Two-Photon Excitation Fluorescence Microscopy with Chromatically Extended Depth of Focus

IDBR：开发具有彩色扩展焦深的高速双光子激发荧光显微镜

• 批准号: 0649866
• 负责人: Zhiwen Liu
• 金额: $ 43.57万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649866&HistoricalAwards=false
• 关键词: IDBR; Development; Speed; Two; Photon

This award is for the development of a faster two-photon fluorescence microscope, with improvements especially in the axial scanning speed. Speed will be increased by wavelength division multiplexing (or chromatic two-photon imaging). Although much progress has been made in improving the lateral scanning speed, a high-speed axial scanning method is still missing. Slow mechanical translation of the objective lens or the specimen itself is still required in order to scan in the axial direction, which limits the 3D imaging speed. As a result, existing instruments have very limited capability to monitor fast biological processes (e.g., transient calcium signals) in 3D and in the x-z or y-z cross-section. The proposed instrument will have a significant impact on 3D microscopy, one of the most important and widely used tools in biology. It will allow researchers to study fast dynamics (e.g., membrane trafficking, chemical waves within or between cells) and provide fundamental understanding of various inter- and intra- cellular processes such as calcium imaging. In addition to its scientific benefits, the project will involve undergraduate researchers as well as graduate students. Woman and minority students will be particularly encouraged to be involved in the program. The investigators will work closely with Penn State organizations such as Women in Engineering to develop outreach education programs for under-represented groups as well as K-12 students.

该奖项旨在表彰更快的双光子荧光显微镜的开发，特别是在轴向扫描速度方面的改进。 速度将通过波分复用（或彩色双光子成像）来提高。虽然在提高横向扫描速度方面取得了很大进展，但仍然缺少高速轴向扫描方法。仍然需要物镜透镜或样本本身的缓慢机械平移以便在轴向方向上扫描，这限制了3D成像速度。结果，现有仪器具有非常有限的监测快速生物过程（例如，瞬时钙信号）。 拟议的仪器将对3D显微镜产生重大影响，3D显微镜是生物学中最重要和最广泛使用的工具之一。 它将使研究人员能够研究快速动力学（例如，膜运输，细胞内或细胞间的化学波），并提供对各种细胞间和细胞内过程的基本理解，如钙成像。除了其科学效益，该项目将涉及本科研究人员以及研究生。将特别鼓励妇女和少数民族学生参与该方案。调查人员将与宾夕法尼亚州立大学的妇女工程等组织密切合作，为代表性不足的群体以及K-12学生制定外展教育计划。