NER: Building Biomimetic Nano-Tracks and Transporters on Target-Recognizing Filamentous Viruses

NER：在目标识别丝状病毒上构建仿生纳米轨道和转运蛋白

• 批准号: 0709287
• 负责人: Chuanbin Mao
• 金额: --
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0709287&HistoricalAwards=false
• 关键词: NER; Building; Biomimetic; Nano; Tracks

Intellectual Merit: The objective of this project is to use a filamentous virus called phage to identify and display peptides that can specifically bind to the inner wall of a microtubule (a protein nanotube) along the length direction and to template the nucleation and elongation of the microtubule that can serve as a nano-track for the kinesin (a motor protein) nano-transporter. This project is the first example of using a virus as a template to assemble biological nanomachines for the fabrication of novel multi-functional nanostructures. The work will result in a virus-in-microtubule nanostructure. This novel nanostructure is based on the virus that can genetically display target-binding peptides at two distal ends, making it possible to position the nanostructure in between two "stations" for controlled alignment of microtubules. At the same time, the microtubule encasing the virus serves as a nano-track for kinesin carrying a cargo to move in the direction defined by the virus alignment. Broader Impacts. This project represents a novel nanofabrication concept, that is, employing biological macromolecules (viruses and proteins) as sources of molecular recognition and mechanic motion to build and assemble biomimetic nanodevices. The resulting nanodevices can transport cargoes in a precise direction as well as with a precise starting and target position. This project can also provide insight about the manipulation of microtubules as well as the complex mechanisms of microtubule nucleation, mitotic spindle organization and positioning in living cells. Thus, it has implications for studies of microtubule function within human cells that is closely related to human diseases such as cancer. The educational activities include an interdisciplinary bionanotechnology curriculum devlopement, community college and high school student involvement, Oklahoma Native American student involvement and dissemination of the results statewide in Oklahoma to increase the public awareness of bionanotechnology.

智力优势：本项目的目的是利用一种被称为噬菌体的丝状病毒来鉴定和展示能够沿着长度方向特异性结合微管（蛋白质纳米管）内壁的肽，并模板化微管的成核和延伸，从而作为驱动蛋白（马达蛋白）纳米转运蛋白的纳米轨道。该项目是第一个使用病毒作为模板组装生物纳米机器以制造新型多功能纳米结构的例子。这项工作将产生一种微管病毒纳米结构。这种新的纳米结构是基于病毒，可以在基因上显示目标结合肽在两个远端，使其有可能将纳米结构定位在两个“站”之间，以控制微管的排列。与此同时，包裹病毒的微管充当了驱动蛋白携带货物的纳米轨道，以沿着病毒排列所定义的方向移动。更广泛的影响。该项目代表了一种新的纳米纤维概念，即利用生物大分子（病毒和蛋白质）作为分子识别和机械运动的来源来构建和组装仿生纳米器件。由此产生的纳米器件可以以精确的方向以及精确的起始和目标位置运输货物。该项目还可以提供有关微管操作以及微管成核，有丝分裂纺锤体组织和活细胞定位的复杂机制的见解。因此，它对人类细胞内微管功能的研究具有重要意义，这与人类疾病如癌症密切相关。教育活动包括跨学科生物纳米技术课程开发，社区学院和高中学生参与，俄克拉荷马州美洲原住民学生参与和结果在俄克拉荷马州全州范围内的传播，以提高公众对生物纳米技术的认识。