Biomineralization and self-assembly of genetically modifiable nanofibers to build bone tissue engineering scaffolds

基因修饰纳米纤维的生物矿化和自组装构建骨组织工程支架

• 批准号: 0854414
• 负责人: Chuanbin Mao
• 金额: $ 38.55万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0854414&HistoricalAwards=false
• 关键词: Biomineralization; self; assembly; genetically; modifiable

This award is funded under the American Recovery and Reinvestment Act of 2009 Public Law 111-5).0854414Mao Intellectual merit This project will advance the fields of bone biomineralization and tissue engineering while promoting teaching, training and learning. A peptide that can specifically not only bind to but also nucleate hydroxylapatite (HAP) nanocrystals will be identified by phage display. Phage that double-displays this binding/nucleating peptide and the cell-adhesion peptide on its surface can be site-specifically biomineralized and selfassemble into a scaffold, which mimics the chemical composition and hierarchical architecture of bone extracellular matrix (ECM) and thus is expected to have biological functions of natural bone ECM. The resultant ECM-like scaffold can support the proliferation and differentiation of mesenchymal stem cells (MSCs) to form bone in vitro. Broader impacts This project will have impacts on materials science, chemistry, biology, bioengineering and medicine. It shows that phage is a good platform for studying the mechanism of biomineralization and can be applied to develop scaffolds for the engineering of other tissues. It will also be integrated into outreach and educational activities and have many benefits. It will facilitate the development of a new curriculum on bionanotechnology at Oklahoma University to educate a new generation of workforce in the field of bionanotechnology. It will allow bioengineering PhD and undergraduate students to be trained to satisfy the urgent need of multidisciplinary talents in bionanotechnology. Community college students will be motivated to pursue higher education. A Native American Nanotechnology Outreach (NANO) program in Oklahoma that has recently been initiated by the PI will be expanded and solidified by partnering with Native American students, high school educators and the Native American Research Center for Health (NARCH) program in Oklahoma. This program will help Oklahoma Native Americans become interested in scientific careers and competitive in job markets. Public awareness of bionanotechnology will be increased in Oklahoma statewide by partnering with the Oklahoma Nanotechnology Initiative, and more Oklahomans will be Speaking of Nano

该奖项是根据《美国复苏和再投资法案》(2009年第111-5号公法)资助的)。一种不仅能与羟基磷灰石(HAP)纳米晶体特异结合，还能与其成核的多肽将通过噬菌体展示进行鉴定。双展示结合/核化肽和细胞黏附肽的噬菌体可以被定点生物矿化并自组装成支架，模拟骨细胞外基质的化学组成和层次结构，从而有望具有天然骨细胞外基质的生物学功能。所制得的类ECM支架可支持间充质干细胞(MSCs)在体外的增殖和分化成骨。该项目将对材料科学、化学、生物学、生物工程和医学产生更广泛的影响。结果表明，噬菌体是研究生物矿化机理的良好平台，可用于构建其他组织工程支架。它还将被纳入外联和教育活动，并有许多好处。它将促进俄克拉荷马大学开发关于生物纳米技术的新课程，以培养生物纳米技术领域的新一代劳动力。它将允许培养生物工程博士和本科生，以满足生物纳米技术多学科人才的迫切需求。社区大学的学生将被激励去接受高等教育。最近由PI在俄克拉何马州发起的美国原住民纳米技术推广(NANO)计划将通过与美国原住民学生、高中教育工作者和俄克拉何马州的美国原住民健康研究中心(NARCH)计划合作来扩大和巩固。这项计划将帮助俄克拉荷马原住民对科学事业产生兴趣，并在就业市场上具有竞争力。通过与俄克拉荷马州纳米技术倡议合作，俄克拉荷马州全州将提高公众对生物纳米技术的认识，更多的俄克拉荷马州人将谈论纳米技术