CAREER: Genetically Modifiable Shape-Tunable Protein Nanotubes as Templates for Controlled Nano-Synthesis and Assembly

职业：基因改造形状可调蛋白质纳米管作为受控纳米合成和组装的模板

• 批准号: 0847758
• 负责人: Chuanbin Mao
• 金额: $ 50万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0847758&HistoricalAwards=false
• 关键词: CAREER; Genetically; Modifiable; Shape; Tunable

ID: MPS/DMR/BMAT(7623) 0847758 PI: Mao, Chuanbin ORG: OklahomaTitle: CAREER: Genetically Modifiable Shape-Tunable Protein Nanotubes as Templates for Controlled Nano-Synthesis and AssemblyINTELLECTUAL MERIT: Precise assembly of nanocomponents and morphological control of nanoassemblies are still of great challenge in nanotechnology. The objective of this proposal is to use a filamentous bio-nanostructure, Salmonella typhimurium bacterial flagellar filament (FF), as a platform to surface-display an ordered array of foreign peptides for directing the synthesis and assembly of inorganic nanomaterials and understanding protein-material interactions. FF is a self-assembling protein nanotube (2 nm in inner diameter, ~15 nm in outer diameter) that can be purified from bacterial cell surface. It is self-assembled (polymerized) from several thousand copies of a single protein called flagellin (monomer). FFs from Salmonella typhimurium bacteria used in this project are unique compared to other biotemplates used earlier in nanotechnology for the following reasons: (1) The surface chemistry is genetically modifiable. (2) Their shapes can be tuned by simply changing solution conditions. (3) Their length can be controlled. (4) They show lyotropic liquid crystalline (LC) self-assembly and their helical forms can self-assemble into a novel LC structure called conical phase. The PI will use Au nanoparticles (AuNPs) and nanorods (AuNRs) as model materials to study the control of their 1D and 3D nano-assembly by individual and LC phase FFs, respectively. He will also use silica as a model material to study the polymorphism of FFs under materials synthesis condition and FF-templated synthesis of inorganic nanomaterials and nanoholes. The project will show how peptide display on FFs as well as polymorphism and LC self-assembly of engineered FFs can direct the nano-synthesis/assembly with a precise control over the spacing, organization and morphology of nanomaterials.BROADER IMPACTS: This project will be integrated into the PI?s educational activities to: (1) develop an interdisciplinary bionanotechnology (BNT) curriculum at the undergraduate/graduate level, (2) train Ph.D. specialists and undergraduate students with multidisciplinary skills in BNT, (3) equip community college (CC) students with BNT knowledge & skills by partnering with CCs, (4) develop a Native American Nanotechnology Outreach (NANO) program by partnering with Native American students, high school (HS) educators and Native American Research Center for Health (NARCH) program in Oklahoma, (5) nurture ?Bio-Nano-Aware? HS students and teachers through outreach to HSs, and (6) organize ?Speaking of Nano? events and disseminate BNT research findings to Oklahoma statewide by partnering with the Oklahoma Nanotechnology Initiative (ONI).

ID：MPS/DMR/BMAT（7623）0847758 主要研究者：毛传斌 ORG：Oklahoma标题：职业：可遗传修饰的形状可调的蛋白质纳米管作为模板控制纳米合成和组装智力优势：纳米组件的精确组装和纳米组装体的形态控制仍然是纳米技术的巨大挑战。 本提案的目的是使用丝状生物纳米结构，鼠伤寒沙门氏菌细菌鞭毛丝（FF），作为一个平台，表面展示有序阵列的外来肽，指导无机纳米材料的合成和组装，并了解蛋白质材料的相互作用。 FF是一种自组装的蛋白质纳米管（内径2 nm，外径约15 nm），可以从细菌细胞表面纯化。 它是由几千个被称为鞭毛蛋白（单体）的单一蛋白质拷贝自组装（聚合）而成。 与纳米技术早期使用的其他生物模板相比，本项目中使用的来自鼠伤寒沙门氏菌的FF是独特的，原因如下：（1）表面化学是可遗传修饰的。(2)它们的形状可以通过简单地改变溶液条件来调整。(3)长度可以控制。(4)它们表现出溶致液晶（LC）自组装，并且它们的螺旋形式可以自组装成称为锥形相的新型LC结构。 PI将使用Au纳米颗粒（AuNPs）和纳米棒（AuNRs）作为模型材料，分别研究单个和LC相FF对其1D和3D纳米组装的控制。 他还将使用二氧化硅作为模型材料，研究材料合成条件下FF的多晶型以及无机纳米材料和纳米孔的FF模板合成。 该项目将展示FF上的肽展示以及工程FF的多态性和LC自组装如何通过精确控制纳米材料的间距、组织和形态来指导纳米合成/组装。更广泛的影响：该项目将被整合到PI？的教育活动：（1）开发一个跨学科的生物纳米技术（BNT）课程在本科/研究生水平，（2）培养博士学位。 通过与社区学院（CC）合作，为社区学院（CC）的学生提供BNT知识技能，（4）通过与俄克拉荷马州的美国原住民学生、高中（HS）教育工作者和美国原住民健康研究中心（NINO）计划合作，开发美国原住民纳米技术推广（NANO）计划，（5）培养？生物纳米感知？HS学生和教师通过推广HS，和（6）组织？关于Nano？通过与俄克拉荷马州纳米技术倡议（ONI）合作，将BNT的研究成果传播到俄克拉荷马州全州。