CAREER: Genetically Modifiable Shape-Tunable Protein Nanotubes as Templates for Controlled Nano-Synthesis and Assembly

ID: MPS/DMR/BMAT(7623) 0847758皮：毛，传宾ORG：奥克拉荷马标题：基因修饰的形状可调蛋白纳米管作为模板控制纳米合成和组装知识价值：纳米组件的精确组装和纳米组件的形态控制仍然是纳米技术的巨大挑战。本研究的目的是利用一种丝状生物纳米结构，即鼠伤寒沙门氏菌鞭毛丝（FF），作为一个平台，在表面展示有序排列的外源肽，以指导无机纳米材料的合成和组装，并了解蛋白质与材料的相互作用。FF是一种可自组装的蛋白纳米管（内径2nm，外径~ 15nm），可从细菌细胞表面纯化。它是由一种叫做鞭毛蛋白（单体）的蛋白质的几千个拷贝自组装（聚合）而成的。与早期在纳米技术中使用的其他生物模板相比，本项目中使用的鼠伤寒沙门氏菌FFs是独一无二的，原因如下：(1)表面化学是可基因修饰的。(2)它们的形状可以通过简单地改变溶液条件来调整。(3)长度可控制。(4)它们具有溶致液晶（LC）的自组装特性，它们的螺旋结构可以自组装成一种新的LC结构，称为锥形相。PI将使用Au纳米颗粒（AuNPs）和纳米棒（AuNRs）作为模型材料，分别研究单个和LC相ff对其1D和3D纳米组装的控制。他还将以二氧化硅为模型材料，研究ff在材料合成条件下的多态性，以及ff模板合成无机纳米材料和纳米孔。该项目将展示肽在FFs上的显示以及工程FFs的多态性和LC自组装如何通过精确控制纳米材料的间距，组织和形态来指导纳米合成/组装。更广泛的影响：本项目将纳入个人利益计划？S教育活动：(1)在本科/研究生阶段开发跨学科的生物纳米技术（BNT）课程，(2)培养具有生物纳米技术多学科技能的博士专家和本科生，(3)通过与社区学院合作，为社区学院（CC）的学生提供生物纳米技术知识和技能，(4)通过与美洲原住民学生合作，开发美洲原住民纳米技术推广（NANO）计划，高中（HS）教育工作者和美国原住民健康研究中心（NARCH）计划，(5)培育？生物纳米意识？高中的学生和老师通过外展到高中，和(6)组织？说到Nano？通过与俄克拉荷马州纳米技术倡议（ONI）合作，将BNT研究成果传播到俄克拉荷马州。

ID: MPS/DMR/BMAT(7623) 0847758 PI: Mao, Chuanbin ORG: OklahomaTitle: CAREER: Genetically Modifiable Shape-Tunable Protein Nanotubes as Templates for Controlled Nano-Synthesis and AssemblyINTELLECTUAL MERIT: Precise assembly of nanocomponents and morphological control of nanoassemblies are still of great challenge in nanotechnology. The objective of this proposal is to use a filamentous bio-nanostructure, Salmonella typhimurium bacterial flagellar filament (FF), as a platform to surface-display an ordered array of foreign peptides for directing the synthesis and assembly of inorganic nanomaterials and understanding protein-material interactions. FF is a self-assembling protein nanotube (2 nm in inner diameter, ~15 nm in outer diameter) that can be purified from bacterial cell surface. It is self-assembled (polymerized) from several thousand copies of a single protein called flagellin (monomer). FFs from Salmonella typhimurium bacteria used in this project are unique compared to other biotemplates used earlier in nanotechnology for the following reasons: (1) The surface chemistry is genetically modifiable. (2) Their shapes can be tuned by simply changing solution conditions. (3) Their length can be controlled. (4) They show lyotropic liquid crystalline (LC) self-assembly and their helical forms can self-assemble into a novel LC structure called conical phase. The PI will use Au nanoparticles (AuNPs) and nanorods (AuNRs) as model materials to study the control of their 1D and 3D nano-assembly by individual and LC phase FFs, respectively. He will also use silica as a model material to study the polymorphism of FFs under materials synthesis condition and FF-templated synthesis of inorganic nanomaterials and nanoholes. The project will show how peptide display on FFs as well as polymorphism and LC self-assembly of engineered FFs can direct the nano-synthesis/assembly with a precise control over the spacing, organization and morphology of nanomaterials.BROADER IMPACTS: This project will be integrated into the PI?s educational activities to: (1) develop an interdisciplinary bionanotechnology (BNT) curriculum at the undergraduate/graduate level, (2) train Ph.D. specialists and undergraduate students with multidisciplinary skills in BNT, (3) equip community college (CC) students with BNT knowledge & skills by partnering with CCs, (4) develop a Native American Nanotechnology Outreach (NANO) program by partnering with Native American students, high school (HS) educators and Native American Research Center for Health (NARCH) program in Oklahoma, (5) nurture ?Bio-Nano-Aware? HS students and teachers through outreach to HSs, and (6) organize ?Speaking of Nano? events and disseminate BNT research findings to Oklahoma statewide by partnering with the Oklahoma Nanotechnology Initiative (ONI).