SBIR Phase I:Manufacturing Platform for Immunoassays

SBIR 第一期：免疫分析制造平台

• 批准号: 0711753
• 负责人: John Anthony
• 金额: --
• 依托单位: Elion Systems Incorporated
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0711753&HistoricalAwards=false
• 关键词: SBIR; Phase; Manufacturing; Platform; Immunoassays

This Small Business Innovation Research (SBIR) Phase I research uses electrospray ionization as a deposition method for rapid and reproducible generation of advanced biosensor substrates. Deposition of bioactive surfaces for immunoassay based sensing elements is usually based on a wet-chemical approach. Unfortunately, the preparation and covalent linkage steps in such wet-chemical approaches can take several days, and are prone to inconsistency due to the elaborate multi-step processes. Electrospray ionization offers the opportunity for a new approach to this problem by direct spray deposition. The ionization process enables direct beam focusing, leading naturally to patterning and array based sensing elements. Electrospray deposition not only promises better control over the assay quality, but also enables production schemes such as continuous roll-to-roll processing. Since the process leverages directed deposition rather than diffusion kinetics from a liquid overlayer, process times of minutes are possible. Preliminary results for detection of pathogenic E. coli O157:H7 bacteria using electrospray deposited immunoassay substrates have demonstrated sensitivities similar to wet chemically prepared samples, and further optimization will be performed. This research will lead to improved methods for production of biosensors that delivers higher quality at a reduced cost to improve the overall utility of biosensors to the country.

这项小型企业创新研究（SBIR）的第一阶段研究使用电喷雾电离作为一种沉积方法，用于快速和可重复地生成先进的生物传感器基板。基于免疫测定的传感元件的生物活性表面沉积通常基于湿化学方法。不幸的是，这种湿化学方法的制备和共价连接步骤可能需要几天时间，而且由于复杂的多步骤过程，容易出现不一致。电喷雾电离为直接喷雾沉积提供了解决这一问题的新途径。电离过程使光束能够直接聚焦，从而自然地产生基于图像化和阵列的传感元件。电喷雾沉积不仅承诺更好地控制分析质量，而且还实现了生产方案，如连续卷对卷处理。由于该工艺利用了定向沉积而不是液体覆盖层的扩散动力学，因此可以在几分钟内完成工艺。使用电喷雾沉积免疫测定底物检测致病性大肠杆菌O157:H7细菌的初步结果表明，该底物的敏感性与湿化学制备的样品相似，将进一步优化。这项研究将改进生物传感器的生产方法，以更低的成本提供更高的质量，从而提高生物传感器对该国的整体效用。