SBIR Phase I:Manufacturing Platform for Immunoassays

这项小型企业创新研究（SBIR）第一阶段研究使用电喷雾离子化作为快速和可重复生成先进生物传感器基底的沉积方法。 用于基于免疫测定的传感元件的生物活性表面的沉积通常基于湿化学方法。不幸的是，这种湿化学方法中的制备和共价连接步骤可能需要几天，并且由于复杂的多步骤过程而易于不一致。电喷雾电离提供了一个新的途径，这个问题的直接喷雾沉积的机会。 电离过程使得能够直接聚焦束，自然地导致基于感测元件的图案化和阵列。 电喷雾沉积不仅保证了对分析质量的更好控制，而且还实现了诸如连续卷对卷处理的生产方案。 由于该工艺利用定向沉积而不是来自液体覆盖层的扩散动力学，因此几分钟的工艺时间是可能的。 病原性E.大肠杆菌O157：H7细菌使用电喷雾沉积的免疫测定底物已被证明与湿化学制备的样品相似的灵敏度，并将进行进一步的优化。这项研究将改进生物传感器的生产方法，以降低成本提供更高的质量，以提高生物传感器对该国的整体效用。

This Small Business Innovation Research (SBIR) Phase I research uses electrospray ionization as a deposition method for rapid and reproducible generation of advanced biosensor substrates. Deposition of bioactive surfaces for immunoassay based sensing elements is usually based on a wet-chemical approach. Unfortunately, the preparation and covalent linkage steps in such wet-chemical approaches can take several days, and are prone to inconsistency due to the elaborate multi-step processes. Electrospray ionization offers the opportunity for a new approach to this problem by direct spray deposition. The ionization process enables direct beam focusing, leading naturally to patterning and array based sensing elements. Electrospray deposition not only promises better control over the assay quality, but also enables production schemes such as continuous roll-to-roll processing. Since the process leverages directed deposition rather than diffusion kinetics from a liquid overlayer, process times of minutes are possible. Preliminary results for detection of pathogenic E. coli O157:H7 bacteria using electrospray deposited immunoassay substrates have demonstrated sensitivities similar to wet chemically prepared samples, and further optimization will be performed. This research will lead to improved methods for production of biosensors that delivers higher quality at a reduced cost to improve the overall utility of biosensors to the country.