SGER: Development of Equipement and Methodology for Simultaneous Measurements of Epithelial Transport and Oxygen Consumption

SGER：同时测量上皮运输和耗氧量的设备和方法的开发

• 批准号: 0714024
• 负责人: Martin Grosell
• 金额: $ 4.7万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-15 至 2008-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0714024&HistoricalAwards=false
• 关键词: SGER; Development; Equipement; Methodology; Simultaneous

The present project seeks to develop equipment and methods to examine energy use by the intestine of marine fish. Marine fish are among the few animals that can drink seawater to avoid dehydration. Humans, by comparison, will die of dehydration if left with seawater as their only water source. The principal investigator of the present research activity has, for a number of years, been studying the physiology of the salt and water transport in the marine fish intestine, an organ which allows these fish to live in the ocean. This research effort has provided preliminary evidence suggesting that the intestine in marine fish requires high amounts of energy to process the ingested seawater. Furthermore, it appears that the metabolic cost of salt and water transport by the fish intestine might be higher than that of the kidney and other salt- and water-transporting organs in other animals. To determine the actual energetic cost associated with salt absorption in the marine fish intestine, development of novel equipment is necessary. The proposed equipment will allow for simultaneous measurements of both intestinal oxygen consumption, a reliable indicator of energy use, and salt transport, by making use of recently developed fluorescence based measurements of oxygen. The principal investigator is working in close collaboration with a company specialized in the development and production of scientific equipment for studies of organ and animal function. The expectation is that the designed equipment will be produced and tested within 12 months after which this novel technology will be made available for other researchers in the United States and the rest of the world.

目前的项目旨在开发设备和方法来检查海洋鱼类肠道的能量使用。海鱼是为数不多的可以通过喝海水来避免脱水的动物之一。相比之下，如果让海水作为人类唯一的水源，人类将会死于脱水。本研究活动的首席研究员多年来一直在研究海洋鱼类肠道中盐和水运输的生理学，肠道是这些鱼类在海洋中生存的器官。这项研究工作提供了初步证据，表明海洋鱼类的肠道需要大量的能量来处理摄入的海水。此外，鱼类肠道运输盐和水的代谢成本可能高于其他动物肾脏和其他盐和水运输器官的代谢成本。为了确定海洋鱼类肠道中盐吸收的实际能量成本，有必要开发新型设备。拟议的设备将允许同时测量肠道耗氧量（能量使用的可靠指标）和盐运输，通过使用最近开发的基于荧光的氧气测量。首席研究员正在与一家专门开发和生产用于器官和动物功能研究的科学设备的公司密切合作。预计设计的设备将在12个月内生产和测试，之后这项新技术将提供给美国和世界其他地区的其他研究人员。