DNA Recognition during DNA Packaging by the Lambda-like Bacteriophages: Sites and Proteins
类 Lambda 噬菌体在 DNA 包装过程中的 DNA 识别:位点和蛋白质
基本信息
- 批准号:0717620
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-08-01 至 2009-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In the large double-stranded DNA viruses, such as the herpes viruses and the tailed bacteriophages, viral DNA is recognized for packaging thru the interactions of a packaging enzyme, called terminase, and specific recognition sites in the viral DNA. In the lambda-like bacteriophages, these recognition sites are located in an approximately 200 bp-long DNA segment called cos. cos contains several subsites, including cosB, the terminase binding site, and cosN, the site where terminase cuts the DNA to make unit-length DNA molecules. This research investigates the function of a newly-discovered subsite located between cosN and cosB. The possibility that the new site includes a DNA bend will be determined. Whether the new site is needed at the start and/or the end of DNA packaging will be studied. The terminase binding site in the cos of the paradigm lambda-like bacteriophage, lambda, is large and complex, with three binding sites for terminase. Because of this complexity, the terminase of lambda forms a complicated DNA-protein complex on cos. Bacteriophage N15 is a lambda-like virus. The terminase binding site for N15 is very simple -- there is only one terminase binding site. A major question is whether N15 terminase forms a complicated DNA-protein complex, or whether N15 recognition differs fundamentally from lambda recognition. Experiments on how N15 terminase binds the cos of N15 will give important insights into DNA recognition in the dsDNA viruses. This research project will provide challenging research experiences to several undergraduates from diverse backgrounds. The work is of general significance for understanding how dsDNA viruses package viral DNA.
在大的双链DNA病毒中,如疱疹病毒和尾噬菌体,病毒DNA通过包装酶(称为末端酶)和病毒DNA中的特异性识别位点的相互作用被识别用于包装。在类噬菌体中,这些识别位点位于大约200 bp长的称为cos的DNA片段中。cos含有几个亚位点,包括cosB,末端酶结合位点,和cosN,末端酶切割DNA以产生单位长度DNA分子的位点。本研究探讨了一个新发现的位于cosN和cosB之间的子位点的功能。将确定新位点包括DNA弯曲的可能性。将研究在DNA包装的开始和/或结束时是否需要新的位点。在典型的类噬菌体λ的cos中的末端酶结合位点是大而复杂的,具有三个末端酶结合位点。由于这种复杂性,λ的末端酶在cos上形成复杂的DNA-蛋白质复合物。噬菌体N15是一种类噬菌体病毒。N15的末端酶结合位点非常简单--只有一个末端酶结合位点。一个主要的问题是N15末端酶是否形成复杂的DNA-蛋白质复合物,或者N15识别是否与λ识别根本不同。N15末端酶如何结合N15的cos的实验将为dsDNA病毒中的DNA识别提供重要的见解。这个研究项目将提供具有挑战性的研究经验,从不同背景的几个本科生。该工作对于理解dsDNA病毒如何包装病毒DNA具有普遍意义。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Michael Feiss其他文献
Insights on Viral DNA Packaging Motor Mechanisms from the Effects of Motor Residue Changes on Single-Molecule Packaging Dynamics
- DOI:
10.1016/j.bpj.2017.11.3203 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Douglas E. Smith;Mariam Ordyan;Damian delToro;Jean Sippy;Michael Feiss - 通讯作者:
Michael Feiss
On maturation of the bacteriophage lambda chromosome
- DOI:
10.1007/bf00267099 - 发表时间:
1973-12-01 - 期刊:
- 影响因子:2.100
- 作者:
Michael Feiss;Thomas Margulies - 通讯作者:
Thomas Margulies
Decision-Making in Bacteriophage Lambda: A View From the Single Phage
- DOI:
10.1016/j.bpj.2009.12.086 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Lanying Zeng;Samuel O. Skinner;Jean Sippy;Michael Feiss;Ido Golding - 通讯作者:
Ido Golding
The bacteriophage λ cohesive end site: Isolation of spacing/substitution mutations that result in dependence on Escherichia coli integration host factor
- DOI:
10.1007/bf00322459 - 发表时间:
1988-04-01 - 期刊:
- 影响因子:2.100
- 作者:
Greg Miller;Michael Feiss - 通讯作者:
Michael Feiss
Michael Feiss的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Michael Feiss', 18)}}的其他基金
Collaborative Research: Mechanisms of Termination of Viral DNA Packaging
合作研究:病毒DNA包装终止机制
- 批准号:
1158495 - 财政年份:2012
- 资助金额:
-- - 项目类别:
Standard Grant
Role of Chromosome Ends in Virus Development
染色体末端在病毒发育中的作用
- 批准号:
7306820 - 财政年份:1973
- 资助金额:
-- - 项目类别:
Standard Grant
相似国自然基金
基于Recognition-VR 虚拟现实的“家庭-社区-医院三向联动”轻度认知障碍防治模式研究
- 批准号:2021JJ60094
- 批准年份:2021
- 资助金额:0.0 万元
- 项目类别:省市级项目
相似海外基金
Amplification of chiral recognition and discrimination among amino-acid-based nanoscale ions during assembly induced by electrostatic interaction
静电相互作用诱导组装过程中氨基酸纳米级离子之间手性识别和辨别的放大
- 批准号:
2309886 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Continuing Grant
Mechanism for CD8+ T cell recognition and removal of senescent tissue cells during ageing
衰老过程中CD8 T细胞识别和清除衰老组织细胞的机制
- 批准号:
BB/Y003365/1 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Research Grant
Investigation of the essential structural and sequence features for the recognition of RNA methylations during post-transcriptional regulation of gene expression
研究基因表达转录后调控过程中识别 RNA 甲基化的基本结构和序列特征
- 批准号:
10796260 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Face learning and recognition during infancy: An examination using naturalistic images
婴儿期的面部学习和识别:使用自然图像的检查
- 批准号:
568009-2022 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Postdoctoral Fellowships
Brain Responses during Facial Expression Recognition and Individual Differences
面部表情识别过程中的大脑反应和个体差异
- 批准号:
22K03057 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigations Into Dynamic DNA Recognition and Processing During Eukaryotic Nucleotide Excision Repair
真核核苷酸切除修复过程中动态 DNA 识别和加工的研究
- 批准号:
2131806 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Continuing Grant
Development of spoken word recognition during early childhood
幼儿时期口语单词识别的发展
- 批准号:
RGPIN-2021-02859 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Discovery Grants Program - Individual
The Developmental Trajectory of Phonological Similarity Effects During Spoken Word Recognition
语音识别中语音相似效应的发展轨迹
- 批准号:
551279-2020 - 财政年份:2020
- 资助金额:
-- - 项目类别:
University Undergraduate Student Research Awards
Pseudouridine-mediated branch site recognition/selection during pre-mRNA splicing
前 mRNA 剪接过程中假尿苷介导的分支位点识别/选择
- 批准号:
10383755 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Pseudouridine-mediated branch site recognition/selection during pre-mRNA splicing
前 mRNA 剪接过程中假尿苷介导的分支位点识别/选择
- 批准号:
10612829 - 财政年份:2020
- 资助金额:
-- - 项目类别: