Identification of a New Component of the Photorespiratory C2 Cycle in Arabidopsis and Analysis of its Function in Metabolism

在我们组的拟南芥EMS突变体的蛋白质缺陷的光呼吸C2循环的重新检查表明存在额外的和尚未确定的组件，这一途径。我们的项目集中于候选基因之一，GLD 1，其突变导致光呼吸条件下的空气敏感表型和严重的甘氨酸积累（萨默维尔和奥格伦，1982）。我们已经将受影响的基因定位在2号染色体的左臂上，该基因不编码任何已知有助于C2周期的酶。我们希望识别基因，然后分析相应蛋白质的特性和代谢功能。这项工作将有助于更全面地了解植物初级代谢的主要途径之一，与光合作用，氮代谢和一碳代谢有关。

A re-examination in our group of Arabidopsis EMS mutants defective in proteins contributing to the photorespiratory C2 cycle indicates the presence of additional and as yet unidentified components of this pathway. Our project focuses on one of the candidate genes, GLD1, whose mutation leads to an air-sensitive phenotype and severe glycine accumulation under photorespiratory conditions (Somerville and Ogren, 1982). We have mapped the affected gene on the left arm of chromosome 2 which does not encode any enzyme known to contribute to the C2 cycle. We want to identify the gene to then analyse the properties and metabolic function of the respective protein. This work will contribute to a more complete knowledge of one of the major pathways of plant primary metabolism that is linked to photosynthesis, nitrogen metabolism, and one-carbon metabolism.