Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification Technology

提案编号：CTS-0553906首席研究员：Zahn，Jeffrey从属关系：宾夕法尼亚州立大学提案标题：用于生物净化技术的两相微流控系统中的电流体不稳定微混合本项目建议研究有机-水两相微相微流动，并创建一种能够执行液-液萃取技术用于生物样品净化的自主装置。将使用荧光染料定位技术和MicroPIV来研究两相流动行为。通过一种新的侧视microPIV三维轮廓重建技术监测，电流体动力学(EHD)不稳定性可以增强两相的混合，从而增加可能发生液体萃取的表面积。该方案将对两相体系和EHD混合的流动行为进行实验研究，并将结果与分析和计算分析进行比较。通过计算建模对EHD不稳定性的建模也将得到扩展。广泛影响这个问题是一个多学科的问题，将来自工程和生物学的工具结合在一起来解决这个问题，这个问题对于任何未来涉及芯片上样品制备的应用都是至关重要的。这项工作整合了跨学科的技术，并研究了一种强大的DNA提取方法，到目前为止，这种方法很少或根本没有受到关注。这项技术将使高度集成的遗传筛查诊断的样品制备成为可能。对于在分析前使用液体萃取分离程序进行样品纯化的任何未来应用，控制两相混合是一个关键障碍。这项技术为医学基因测试、高通量DNA测序、生物制剂检测和水质监测等不同领域实现了现场生物样本收集和分析的重大前景。在大学一级的科学教育中，将特别重视对代表性不足群体的教育推广。这些活动将增强新兴的生物微机械和纳米生物技术领域的基础知识，并培训该领域的年轻科学家、工程师和教育工作者。

Proposal Number: CTS-0553906Principal Investigator: Zahn, JeffreyAffiliation: Pennsylvania State UniversityProposal Title: Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification TechnologyThis project proposes to study organic-aqueous two phase microflows, and create an autonomous device capable of performing liquid-liquid extraction techniques for biological sample purification.Intellectual MeritThe work will extend the current understanding and manipulation of multiphase flows in microgeometries through experimental, analytical and computational approaches. Two phase flow behavior will be studied using a fluorescent dye localization technique and microPIV. The flow is destabilized by an electrohydrodynamic (EHD) instability to enhance mixing of the two phases to increase the surface area over which liquid extraction may occur, monitored by a novel sideview microPIV technique for three dimensional profile reconstruction. The proposal will experimentally study the flow behavior of the two phase system and EHD mixing and will compare the results to analytical and computational analysis. The modeling of EHD instabilities by computational modeling will also be extended.Broader ImpactThe problem is multidisciplinary, bringing together tools from engineering and biology to attack a problem that is crucial for any future applications involving on-chip sample preparations. This work integrates interdisciplinary technologies and investigates a powerful approach to DNA extraction that has received little or no attention to date. This technique will enable sample preparation for highly integrated genetic screening diagnostics. Controlling two-phase mixing is a key hurdle for any future application that uses a liquid extraction separation procedure for sample purification prior to analysis. This technique holds major promise to enabling on-site biological sample collection and analysis for areas as disparate as medical genetic testing, high throughput DNA sequencing, biowarfare agent detection, and water quality monitoring. Special emphasis will be placed on educational outreach to under-represented groups in college level science education. These activities will enhance fundamental knowledge in the emerging field of BioMEMS and nanobiotechnology as well as train young scientists, engineers and educators in the area.