SBIR Phase II: Novel Labeling Method for Multicolor Fluorescence in situ Hybridization (FISH) Probes

这个小企业创新研究（SBIR）第二阶段研究项目旨在进一步开发一组寡核苷酸荧光原位杂交（FISH）探针，用于进行植入前遗传学诊断（PGD）和检测用于体外受精（IVF）方案的卵子中的非整倍体。 与目前可用的基因组DNA衍生的探针相比，使用寡核苷酸提供了诸如增强的特异性和灵敏度、更短的杂交时间以及降低的制造成本的优点。 该公司计划开发的检测板将涵盖8条已知特别容易发生缺失和重排的染色体，并将允许同时检测可能与它们相关的任何异常。用于检测植入前胚胎遗传异常的探针组的开发将提高IVF程序的成功率，从而降低与之相关的财务和情感成本。 此外，所提出的标记方法的使用可用于PGD以外的各种领域，包括基础研究、临床诊断和细胞遗传学检测。

This Small Business Innovation Research (SBIR) Phase II research project aims to further develop a panel of multicolor oligonucleotide fluorescence in situ hybridization (FISH) probes for performing preimplantation genetic diagnosis (PGD) and detecting aneuploidies in eggs used for in vitro fertilization (IVF) protocols. The use of oligonucleotides offers advantages such as enhanced specificity and sensitivity, shorter hybridization times as well as a reduction in manufacturing cost when compared to currently available genomic DNA derived probes. The panel that the company plans to develop will cover 8 chromosomes known to be particularly susceptible to deletions and rearrangements and would allow simultaneous detection of any abnormalities that may be associated with them.The development of a panel of probes for the detection of genetic abnormalities in preimplantation embryos will increase the success rate of IVF procedures and thus reduce the financial and emotional cost associated with them. Moreover, use of the proposed labeling method can be useful in a variety of areas outside of PGD, including basic research, clinical diagnostics and cytogenetic testing.