Collaborative Research: 2010 Arabidopsis: Cellular and Subcellular Resolution of the Tryptophan-Related Pathways

合作研究:2010 拟南芥:色氨酸相关途径的细胞和亚细胞分辨率

基本信息

  • 批准号:
    0725149
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-08-01 至 2011-07-31
  • 项目状态:
    已结题

项目摘要

The pathways leading to the indolic amino acid tryptophan are of critical importance because tryptophan is required in animal and human diets and because indolic compounds are used as intercellular signals in plants and animals. In addition, indolic pathways reside at the interface of primary and secondary metabolism and function as a node in dynamic metabolic processes that may be predictive of the metabolic status of individual cell types. The objective of this project is to demonstrate the potential of metabolite quantification or profiling methods (metabolomics) to provide insight at the single cell level into the cellular and metabolic consequences of disrupting genes involved in tryptophan biosynthesis. This approach will answer important questions about differences in metabolism between cells that are in close proximity to one another, but which differ in their origin, function or ultimate fate. The project will take advantage of approaches that have been effective when applied to cell-specific transcript profiling, namely sorting of cells with fluorescent protein-tagged fusion proteins in specific cell types, and will apply them to targeted metabolite profiling on a submicro-scale. Tryptophan biosynthesis is an excellent metabolic pathway for such studies not only because the pathway exists at the interface between primary and secondary metabolism, but also because increased tryptophan levels are a potentially valuable crop plant trait and a target for metabolic engineering for animal and human health. A project website (http://www.auxin.org) will detail the specific objectives and progress of the project, including metabolite profiling data. Mutant lines will be deposited at the ABRC.Broader Impacts: The major objective of this project is to advance the methodology of metabolomics (metabolite quantification) at the tissue and cellular levels to the point that it is routinely available to the broader plant community as part of the functional genomics toolbox. The PIs will develop a workshop to disseminate metabolomics methods to the broader scientific community. This project will train postdoctoral fellows, who will be well-positioned to continue the development and application of metabolomics as a genomics tool. The project will interface with on-going activities to recruit and train members of underrepresented groups.The activities meet the goals of the 2010 Project by addressing a recognized need to focus on metabolite profiling in order to characterize the localizome, that is, information about gene and protein expression patterns and metabolite concentrations and flux at the organ, tissue, cellular and/or subcellular levels in the living plant. As noted in the 2010 Project mid-course assessment, knowledge of small molecule biology in Arabidopsis is not only fundamental for a holistic understanding of plant biology, but also in the context of plants as a source of food for both humans and animals as well as sources of pharmaceuticals. As an essential amino acid, tryptophan biosynthesis is broadly relevant, and the approaches developed will allow the study of any metabolite network in plants.
由于色氨酸在动物和人类的饮食中都是必需的,并且吲哚类化合物被用作植物和动物的细胞间信号,因此通向吲哚类氨基酸色氨酸的途径是至关重要的。此外,吲哚通路位于初级和次级代谢的界面,是动态代谢过程中的一个节点,可以预测个体细胞类型的代谢状态。该项目的目的是证明代谢物定量或分析方法(代谢组学)的潜力,以在单细胞水平上深入了解破坏参与色氨酸生物合成的基因的细胞和代谢后果。这种方法将回答关于细胞之间代谢差异的重要问题,这些细胞彼此接近,但它们的起源,功能或最终命运不同。该项目将利用在应用于细胞特异性转录分析时有效的方法,即在特定细胞类型中使用荧光蛋白标记的融合蛋白对细胞进行分类,并将其应用于亚微观尺度上的靶向代谢物分析。色氨酸生物合成是这类研究的一个很好的代谢途径,不仅因为该途径存在于初级和次级代谢的界面,而且因为增加色氨酸水平是一种潜在的有价值的作物植物性状,也是动物和人类健康代谢工程的目标。项目网站(http://www.auxin.org)将详细介绍项目的具体目标和进展,包括代谢物分析数据。突变株将被存放在ABRC。更广泛的影响:该项目的主要目标是在组织和细胞水平上推进代谢组学(代谢物量化)的方法,使其作为功能基因组学工具箱的一部分常规应用于更广泛的植物群落。pi将举办一个研讨会,向更广泛的科学界传播代谢组学方法。该项目将培养博士后,他们将很好地继续发展和应用代谢组学作为基因组学工具。该项目将与正在进行的招募和培训代表性不足群体成员的活动相结合。这些活动满足了2010年项目的目标,解决了关注代谢物分析的公认需求,以表征本地化组,即有关基因和蛋白质表达模式的信息,以及活植物中器官、组织、细胞和/或亚细胞水平的代谢物浓度和通量。正如2010年项目中期评估中所指出的那样,了解拟南芥的小分子生物学不仅是全面了解植物生物学的基础,而且在植物作为人类和动物的食物来源以及药物来源的背景下也是如此。作为一种必需氨基酸,色氨酸的生物合成具有广泛的相关性,所开发的方法将允许研究植物中任何代谢物网络。

项目成果

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Jerry Cohen其他文献

Jerry Cohen的其他文献

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{{ truncateString('Jerry Cohen', 18)}}的其他基金

The relationship between tryptophan and pyridine nucleotide metabolism in plants
植物中色氨酸与吡啶核苷酸代谢的关系
  • 批准号:
    2225057
  • 财政年份:
    2022
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
EAGER: Defining the function and mechanism of YUC1 in tryptophan-dependent indole-3-acetic acid metabolism
EAGER:定义YUC1在色氨酸依赖性吲哚-3-乙酸代谢中的功能和机制
  • 批准号:
    1203438
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Functional Analysis of an Indoleacyl-Modified Protein From Strawberry
草莓吲哚酰基修饰蛋白的功能分析
  • 批准号:
    0820940
  • 财政年份:
    2008
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
SGER: A Novel Protein-linked Route for the Biosynthesis of Indole-3-acetic Acid in Maize Endosperm
SGER:玉米胚乳中吲哚-3-乙酸生物合成的新型蛋白连接途径
  • 批准号:
    0643845
  • 财政年份:
    2006
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Global Measurement of Turnover of Plant Proteins
植物蛋白周转率的全球测量
  • 批准号:
    0606666
  • 财政年份:
    2006
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Arabidopsis 2010: International Research Experience for Senior Graduate Students
拟南芥 2010:高年级研究生的国际研究经验
  • 批准号:
    0529918
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
US-Germany Workshop on Arabidopsis Functional Genomics, June 29-30, 2002, Seville Spain
美德拟南芥功能基因组学研讨会,2002 年 6 月 29-30 日,西班牙塞维利亚
  • 批准号:
    0224476
  • 财政年份:
    2002
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Synergistic Approaches to Understanding Auxin Metabolism
了解生长素代谢的协同方法
  • 批准号:
    8617171
  • 财政年份:
    1987
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
1978 Science Faculty Professional Development Program
1978年理学院专业发展计划
  • 批准号:
    7819068
  • 财政年份:
    1978
  • 资助金额:
    --
  • 项目类别:
    Standard Grant

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