Functional Analysis of an Indoleacyl-Modified Protein From Strawberry

奖项：IOS-0820940。主要研究者：Gardner，加里M.职务名称：草莓果实是研究相邻组织之间信号传导的经典系统，因为花托组织的扩张依赖于瘦果释放的生长素。在过去的几年中，草莓属和蔷薇科其他成员的重要遗传资源已经开发，使得二倍体草莓现在是果实组织内激素信号传导研究的分子表征的重要参考物种。吲哚-3-乙酸（IAA）以游离和结合形式存在于植物中。在缀合的IAA形式中，存在一类独特的蛋白质，其中IAA是直接连接到多肽结构的辅基。第一个基因是一个含有IAA辅基的豆类蛋白基因，已被克隆，并在拟南芥中鉴定了一个相关蛋白和几个较小的肽。该项目的工作重点是在草莓瘦果和花托组织中发现的一种独特的IAA修饰蛋白，该蛋白已被证明是ATP合酶的β亚基。这是第一个具有预测功能的IAA蛋白。虽然我们有一个普遍的兴趣，在了解植物蛋白质的吲哚酰化，目前的项目的目标是集中在一个吲哚酰基修饰的线粒体F1-ATP合酶从二倍体草莓，草莓。该项目将侧重于两个主要目标：1。使用质谱工具来确定IAA附着的位点;以及2.确定IAA修饰对ATP合酶的功能。该项目的更广泛影响如下。该项目将直接支持一名博士后科学家和一名研究生，他们将参与该项目，该项目除了植物生物化学和分子生物学之外，还需要使用蛋白质和小分子质谱分析的现代技术来回答植物生物学中的基本问题。它还将为其他对新型天然修饰肽研究和植物激素代谢研究感兴趣的人提供资源，无论是作为宿主实验室的访问者还是通过将方法转移到自己的设施。

Award: IOS-0820940. Principle Investigator: Gardner, Gary M. Title: Functional Analysis of an Indoleacyl-Modified Protein From StrawberryAward AbstractStrawberry fruit are a classic system for studies of the signaling between adjacent tissues as the expansion of the receptacle tissue is dependent on auxin released from the achenes. Within the last several years significant genetic resources for Fragaria and other members of the Rosaceae have been developed such that the diploid strawberry is now an important reference species for molecular characterization of studies of hormonal signaling within fruit tissues. Indole-3-acetic acid (IAA) is found in plants in both free and conjugated form. Among the conjugated IAA forms, there exists a unique class of proteins where IAA is a prosthetic group attached directly to the polypeptide structure. The first gene, for a bean protein with an IAA prosthetic group, has been cloned and a related protein and several smaller peptides were identified in Arabidopsis. The work in this project focuses on a unique IAA-modified protein found in the achenes and receptacle tissue of strawberry that has been shown to be the beta subunit of ATP synthase. This is the first IAA-protein with a predicted function to be described. While we have a general interest in understanding indoleacylation of plant proteins, the goals of the current project are focused on an indoleacyl-modified mitochondrial F1-ATP synthase from the diploid strawberry, Fragaria vesca. The project will focus on two main goals: 1. the use of mass spectral tools for determination of the site of IAA attachment; and 2. determination of the function of the IAA-modification on the ATP synthase.Broader impacts of the project are as follows. This project will directly support one postdoctoral scientist and one graduate student who will be involved in this project that requires, in addition to plant biochemistry and molecular biology, specific expertise in the use of modern techniques of protein and small molecule mass spectrometric analysis to answer fundamental questions in plant biology. It will also provide a resource for others interested in studies of novel native modified peptides and studies of plant hormone metabolism, either as visitors to the host laboratory or by transfer of the methods to their own facility.