Functional Analysis of an Indoleacyl-Modified Protein From Strawberry

草莓吲哚酰基修饰蛋白的功能分析

• 批准号: 0820940
• 负责人: Jerry Cohen
• 金额: $ 22.91万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0820940&HistoricalAwards=false
• 关键词: Functional; Analysis; Indoleacyl; Modified; Protein

Award: IOS-0820940. Principle Investigator: Gardner, Gary M. Title: Functional Analysis of an Indoleacyl-Modified Protein From StrawberryAward AbstractStrawberry fruit are a classic system for studies of the signaling between adjacent tissues as the expansion of the receptacle tissue is dependent on auxin released from the achenes. Within the last several years significant genetic resources for Fragaria and other members of the Rosaceae have been developed such that the diploid strawberry is now an important reference species for molecular characterization of studies of hormonal signaling within fruit tissues. Indole-3-acetic acid (IAA) is found in plants in both free and conjugated form. Among the conjugated IAA forms, there exists a unique class of proteins where IAA is a prosthetic group attached directly to the polypeptide structure. The first gene, for a bean protein with an IAA prosthetic group, has been cloned and a related protein and several smaller peptides were identified in Arabidopsis. The work in this project focuses on a unique IAA-modified protein found in the achenes and receptacle tissue of strawberry that has been shown to be the beta subunit of ATP synthase. This is the first IAA-protein with a predicted function to be described. While we have a general interest in understanding indoleacylation of plant proteins, the goals of the current project are focused on an indoleacyl-modified mitochondrial F1-ATP synthase from the diploid strawberry, Fragaria vesca. The project will focus on two main goals: 1. the use of mass spectral tools for determination of the site of IAA attachment; and 2. determination of the function of the IAA-modification on the ATP synthase.Broader impacts of the project are as follows. This project will directly support one postdoctoral scientist and one graduate student who will be involved in this project that requires, in addition to plant biochemistry and molecular biology, specific expertise in the use of modern techniques of protein and small molecule mass spectrometric analysis to answer fundamental questions in plant biology. It will also provide a resource for others interested in studies of novel native modified peptides and studies of plant hormone metabolism, either as visitors to the host laboratory or by transfer of the methods to their own facility.

奖项：iOS-0820940。主要研究者：Gardner，Gary M.标题：草莓吲哚修饰蛋白质的功能分析奖摘要草莓果实是研究相邻组织之间信号的经典系统，因为花托组织的扩张依赖于瘦果释放的生长素。在过去的几年里，已经开发了大量的草莓和蔷薇科其他成员的遗传资源，使得二倍体草莓现在成为研究果实组织内激素信号的分子特征的重要参考物种。吲哚-3-乙酸(IAA)以游离态和结合态存在于植物体内。在结合的IAA形式中，存在着一类独特的蛋白质，其中IAA是直接连接到多肽结构上的修复基团。第一个基因是含有IAA修复基团的豆蛋白，已在拟南芥中克隆出相关蛋白和几个较小的多肽。本项目的工作集中在草莓瘦果和花托组织中发现的一种独特的IAA修饰蛋白，该蛋白已被证明是ATP合成酶的β亚基。这是第一个被描述的具有预测功能的IAA蛋白。虽然我们对了解植物蛋白的吲哚酰化有普遍的兴趣，但本项目的目标是从二倍体草莓Fragaria Vesca中获得吲哚酰化修饰的线粒体F1-ATP合成酶。该项目将集中于两个主要目标：1.使用质谱学工具确定IAA的连接位置；2.确定IAA修饰对ATP合成酶的功能。该项目的广泛影响如下。这个项目将直接支持一名博士后科学家和一名研究生，他们将参与这个项目，该项目除了需要植物生物化学和分子生物学外，还需要使用现代蛋白质和小分子质谱分析技术来回答植物生物学的基本问题的专门知识。它还将为其他对新的天然修饰多肽和植物激素代谢研究感兴趣的人提供资源，无论是作为东道主实验室的访问者，还是通过将方法转移到他们自己的设施。