Integrated Optical Microfluidic Chips for On-Site Fluorescence-Based Assays

用于现场荧光检测的集成光学微流控芯片

• 批准号: 0725812
• 负责人: David Klotzkin
• 金额: --
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0725812&HistoricalAwards=false
• 关键词: Integrated; Optical; Microfluidic; Chips; Site

The objective of this research is to develop integrated disposable microfluidic systems for fluorescence-based assays for point-of-care diagnostics and point-of-use environmental applications. The approach will be to combine microfluidic chips with the excitation light and detector using organic light emitters and organic photovoltaics into a single compact and economical medical assay tool. Integrated filtering which is necessary for fluorescence will be done using polarization rather than spectral filtering. The integrated device will be independent of wavelength and is expected to be used with multiple fluorescent dyes for simultaneous detection. This will miniaturize and integrate the analysis and sample manipulation capabilities.The intellectual merit of this work are in the integration of organic photonics and biomedical microfluidics to realize an economical device with integrated microfluidics, organic excitation sources, and organic detectors, for compact, disposable, and quantitative fluorescence based assays. These systems would replace laboratory-based analysis using fixed, specialized microscopes, by substituting compact, stand-alone devices using inexpensive light emitters and detectors made from small-molecule organics and polymers. Broader impacts include the widespread availability of economical quantitative analysis system for detection of low levels of analytes on-site with applications to medical diagnosis, environmental monitoring, and homeland defense. Undergraduate students from under-represented groups will be recruited through our universities Rowe Center for Women and the Emerging Ethnic Engineers program and funded through the REU program. Material developed will be incorporated into our ongoing course on ?Biosensors and BioMEMS? which focuses on microfluidic sensors and systems for biomedical and environmental applications.

本研究的目的是开发集成的一次性微流体系统，用于基于荧光的检测，用于点护理诊断和点使用环境应用。该方法将微流控芯片与使用有机光发射器和有机光伏的激发光和检测器相结合，形成一个单一的紧凑和经济的医学分析工具。荧光所必需的综合滤波将使用偏振而不是光谱滤波来完成。该集成装置将独立于波长，并有望与多种荧光染料同时检测。这将使分析和样品操作能力小型化和集成。这项工作的智力优势在于有机光子学和生物医学微流体的集成，实现了集成微流体、有机激发源和有机检测器的经济装置，用于紧凑、一次性和定量的荧光分析。这些系统将取代使用固定的、专门的显微镜的实验室分析，取而代之的是使用廉价的光发射器和由小分子有机物和聚合物制成的探测器的紧凑、独立的设备。更广泛的影响包括广泛使用经济的定量分析系统，用于现场检测低水平的分析物，并应用于医疗诊断，环境监测和国土防御。来自弱势群体的本科生将通过我们大学的罗妇女中心和新兴族裔工程师项目招收，并由REU项目资助。所开发的材料将纳入我们正在进行的课程？生物传感器和生物ems ？专注于生物医学和环境应用的微流体传感器和系统。