Integrated optical sensors for microfluidic free-flow electrophoresis

用于微流体自由流动电泳的集成光学传感器

• 批准号: 192305443
• 负责人: Professor Dr. Stefan Nagl
• 金额: --
• 依托单位: Department of Chemistry
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/192305443?language=en
• 关键词: Integrated; optical; sensors; microfluidic; free

Miniaturization of analytical techniques on microchips aims for faster chemical analysis on smaller scales; in order to conserve resources, enable the investigation of biological and medical samples that are available only in very small amounts and perform analysis where it is needed. Electrophoretic separation procedures on microchips are capable of sample analysis with high resolution and sensitivity but are complex to develop and read out because many relevant parameters can only be observed indirectly. Integration of optical sensors in microfluidic separation procedures aims to overcome these constraints.In the previous project period the integration of planar optical chemical sensors and sensor arrays in microfluidic free flow electrophoresis (micro-FFE) platforms was demonstrated for the first time. Environmental parameters such as pH can now be analyzed spatially resolved and in real time in micro-FFE.In this project, two important parameters in FFE, the pH gradient in isoelectric focusing (IEF) and temperature differences produced by Joule heating in FFE (IEF) are investigated by chip-integrated optical sensors and the results are used for improvement and optimization of microanalytical separation procedures. Initially this will be performed on selected protein mixtures and the results are later adapted for analysis of real world samples. This will be exemplarily demonstrated on proteins and organelles from cell culture lysates. To this end, detection methods will be developed for FFE in order to provide for simultaneous recording of both labeled analytes and sensor layer without interferences. These are based on ratiometric multispectral data acquisition combined with fluorescence lifetime measurements.In further project sections these analytical procedures are then extended. In order to gain more information from chip-integrated sensor layers, simultaneous spatially resolved dual sensing of pH and temperature in micro electrophoresis will be investigated. The analysis of native, unlabeled proteins in micro-FFE with integrated sensor layers will be developed. In the final part micro-FFE chips with integrated chemical sensors will be coupled to a microfluidic reactor in order to enable both chemical synthesis and subsequent electrophoretic analysis on a microchip using integrated optical chemical sensor layers.

微型芯片分析技术的小型化旨在更快地进行更小规模的化学分析;为了节省资源，能够对只有非常少量的生物和医学样品进行研究，并在需要时进行分析。微芯片上的电泳分离程序能够以高分辨率和灵敏度进行样品分析，但是开发和读取复杂，因为许多相关参数只能间接观察。在微流控分离过程中集成光学传感器旨在克服这些限制。在上一个项目期间，首次展示了在微流控自由流电泳（micro-FFE）平台中集成平面光学化学传感器和传感器阵列。环境参数如pH值，现在可以分析空间分辨和真实的micro-FFE.在这个项目中，两个重要的参数FFE，pH梯度等电聚焦（IEF）和焦耳加热FFE（IEF）产生的温度差进行了研究芯片集成的光学传感器和结果用于改进和优化的微分析分离程序。最初，这将在选定的蛋白质混合物上进行，随后将结果用于分析真实的世界样品。这将在来自细胞培养物裂解物的蛋白质和细胞器上示例性地证明。 为此，将开发用于FFE的检测方法，以便在没有干扰的情况下同时记录标记的分析物和传感器层。这些方法是基于比率多光谱数据采集结合荧光寿命测量。在进一步的项目部分，这些分析程序，然后扩展。为了获得更多的信息，从芯片集成的传感器层，同时空间分辨的pH值和温度的双传感微电泳将进行研究。将开发具有集成传感器层的微型FFE中的天然未标记蛋白质的分析。在最后一部分中，具有集成化学传感器的微FFE芯片将耦合到微流体反应器，以便使用集成的光学化学传感器层在微芯片上实现化学合成和随后的电泳分析。

项目成果

Label-free microfluidic free-flow isoelectric focusing, pH gradient sensing and near real-time isoelectric point determination of biomolecules and blood plasma fractions.

• DOI: 10.1039/c5an01345c
• 发表时间: 2015-10
• 期刊: The Analyst
• 作者: E. Poehler;C. Herzog;Carsten Lotter;Simon A. Pfeiffer;D. Aigner;T. Mayr;S. Nagl

In-line monitoring of pH and oxygen during enzymatic reactions in off-the-shelf all-glass microreactors using integrated luminescent microsensors

• DOI: 10.1007/s00604-016-2021-2
• 发表时间: 2017-02-01
• 期刊: MICROCHIMICA ACTA
• 影响因子: 5.7
• 作者: Pfeiffer, Simon A.;Borisov, Sergey M.;Nagl, Stefan
• 通讯作者: Nagl, Stefan

Development of microscopic time‐domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free‐flow isoelectric focusing

开发用于微流体自由流等电聚焦中 pH 监测的微观时域双寿命参考发光检测

• DOI: 10.1002/elsc.201400081
• 发表时间: 2015
• 期刊: Engineering in Life Sciences
• 影响因子: 2.7
• 作者: E. Poehler;C. Herzog;M. Suendermann;S.A. Pfeiffer;S. Nagl
• 通讯作者: S. Nagl