SGER: Inexpensive Nitrate Nutrient Sensor Arrays

SGER：廉价的硝酸盐营养传感器阵列

• 批准号: 0726956
• 负责人: Malcolm Stokes
• 金额: --
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0726956&HistoricalAwards=false
• 关键词: SGER; Inexpensive; Nitrate; Nutrient; Sensor

The PIs have requested a Small Grant for Exploratory Research (SGER) to develop an inexpensive nitrate sensor array. This proposal requests funds to fabricate and test a new transducer for nitrate sampling that could be incorporated into the successful BOA design or work as part of an autonomous instrument. The PIs will use the enzyme, nitrate reductase, encapsulated within a sol-gel matrix as a nitrate specific and robust optical sensor for dissolved nutrients. The use of this enzyme for nutrient sensing has been proven in the laboratory and the optoelectronics required to couple this biosensor technology to the proven BOA design will produce a robust sensor of great utility. The sol-gel sensor can work in a broad range of pH, has nMol sensitivity, is impervious to saltwater interferents, resists leaching and should be fabricated at a fraction of the cost of commercially available autonomous instruments. They will fabricate a nitrate sensor for testing as an oceanographic instrument. Data from the device will be compared to a commercially available nitrate sensor moored in tandem along with water samples processed for nitrate content by the SIO analytical facility. Broader ImpactsThere is a need for low-cost, precision instruments in both terrestrial and marine field science. In order to synchronously sample environmental factors like nutrients, temperature, pressure, or conductivity on spatial scales of meters to hundreds of meters requires hundreds of sensors which is both prohibitively expensive and logistically difficult with the current technology available to most researchers. The design of the sensors and the data generated by BOA will be shared with other researchers through publication in the appropriate literature and will be available for use by other research teams. The array fabrication and testing facility will include the participation of undergraduate interns from UCSD and the SIO summer research fellowship program that provides hands on experience in oceanography to students from around the country. Stokes and Deane also work closely with local high school educators to mentor young students in marine science projects.

PI已经申请了探索性研究（SGER）的小额赠款，以开发廉价的硝酸盐传感器阵列。该提案要求提供资金，用于制造和测试一种用于硝酸盐采样的新传感器，该传感器可以被纳入成功的BOA设计或作为自主仪器的一部分工作。PI将使用封装在溶胶-凝胶基质中的酶硝酸还原酶作为溶解营养素的硝酸盐特异性和鲁棒性光学传感器。使用这种酶进行营养感测已在实验室中得到证明，将这种生物传感器技术与经过验证的BOA设计相结合所需的光电子学将产生具有巨大实用性的强大传感器。溶胶-凝胶传感器可以在广泛的pH值范围内工作，具有nMol灵敏度，不受盐水干扰，抗浸出，并且应该以商业上可用的自主仪器的一小部分成本制造。他们将制造一种硝酸盐传感器，作为海洋学仪器进行测试。来自该装置的数据将与一个市售硝酸盐传感器进行比较，该传感器沿着停泊在SIO分析设施处理硝酸盐含量的水样中。陆地和海洋领域科学都需要低成本的精密仪器。为了同步采样环境因素，如营养物质，温度，压力，或电导率在空间尺度上的米到数百米需要数百个传感器，这是非常昂贵的和后勤困难与目前的技术可供大多数研究人员。传感器的设计和BOA生成的数据将通过在适当的文献中发表与其他研究人员共享，并将可供其他研究团队使用。阵列制造和测试设施将包括来自UCSD的本科实习生和SIO夏季研究奖学金计划的参与，该计划为来自全国各地的学生提供海洋学方面的实践经验。斯托克斯和迪恩还与当地高中教育工作者密切合作，指导年轻学生参与海洋科学项目。