Design and Characterization of Cyanobacterial Bioreporters to measure Nutrient Availability in Marine Systems

蓝藻生物报告仪的设计和表征，用于测量海洋系统中的营养物质可用性

• 批准号: 0727644
• 负责人: Robert McKay
• 金额: $ 35.61万
• 依托单位: Bowling Green State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0727644&HistoricalAwards=false
• 关键词: Design; Characterization; Cyanobacterial; Bioreporters; measure

For decades, our ideas on nutrient availability in aquatic ecosystems have been informed largely by chemical measurement of dissolved elements. While providing a reasonable proxy, it tells us little about nutrient bioavailability, a feature particularly applicable to nutritive trace metals such as iron (Fe) but also relevant to macronutrients like phosphorus (P). Distinguishing between biologically available and refractory forms of a nutrient is often cited as particularly challenging by the oceanographic community.In this project, researchers at Bowling Green State University will continue the development and characterization of luminescent cyanobacterial bioreporters to offer a rapid assessment of nutrient bioavailability from natural waters. In their previous NSF-sponsored research, they demonstrated the efficacy of this approach toward determining Fe availability in freshwater systems. Now, they will apply a parallel approach to assess nutrient availability in marine systems. Their recent success in developing a luminescent whole-cell cyanobacterial bioreporter for measuring Fe availability in diverse marine environments (Boyanapalli et al. 2007) has provided important proof of concept. They now hope to expand the use of bioreporters to the ocean milieu. The project will focus on the development of Fe- and P-responsive cyanobacterial bioreporters. Acknowledging widespread recognition that Fe distribution and availability is significant in terms of aquatic production, especially associated with ?high nutrient, low chlorophyll? (HNLC) regions of the ocean, they will construct a series of complementary Fe-responsive bioreporters capable of sensing a broad range of bioavailable Fe. Similarly, they plan to construct P-responsive bioreporters having the ability to discriminate between species of bioavailable DIP and DOP. Whereas P limitation is a central tenet of modern day limnology, it has only recently been afforded more widespread attention among oceanographers. Yet, low surface levels of P are associated with much of the world oceans. In particular, the western regions of the North Atlantic Ocean are P-limited as is the Mediterranean Sea. A field component of this proposal involves participation on a cruise of opportunity in the Sargasso Sea. . In terms of broader impacts, the project will feature a special educational outreach . Since 2004, BGSU has been involved in a partnership with Lorain County Community College (LCCC) through their innovative University Partnership (UP) program. The UP was created to provide the residents of Lorain and surrounding counties, which form part of Ohio?s economically-depressed ?rust belt?, an opportunity to receive bachelors degrees while attending LCCC. Through the UP, institutions such as BGSU provide faculty and curriculum to augment course offerings at LCCC through distance learning. To facilitate research, LCCC students will be paired with a BGSU faculty co-advisor who works with a counterpart at LCCC so that the student can conduct his/her project using LCCC lab facilities.

几十年来，我们对水生生态系统中营养物质可用性的看法主要是通过溶解元素的化学测量来了解的。虽然提供了一个合理的替代，但它告诉我们的营养生物利用度很少，这一特征特别适用于营养微量金属，如铁（Fe），但也与磷（P）等常量营养素有关。区分生物可利用和难降解形式的营养素通常被认为是海洋学界特别具有挑战性的。在这个项目中，鲍林绿色州立大学的研究人员将继续开发和表征发光蓝藻生物报告者，以快速评估自然沃茨的营养素生物利用度。在他们之前的NSF赞助的研究中，他们证明了这种方法在确定淡水系统中Fe可用性方面的有效性。 现在，他们将采用一种平行的方法来评估海洋系统中的营养物质可用性。他们最近成功开发了一种发光的全细胞蓝藻生物报告器，用于测量不同海洋环境中的铁可用性（Boyanapalli等人，2007年），这为概念提供了重要的证据。他们现在希望将生物报告者的使用扩展到海洋环境。该项目将集中在铁和磷响应蓝藻生物报告的发展。承认铁的分布和可用性在水产生产方面是重要的，特别是与？高营养低叶绿素？（HNLC）区域的海洋，他们将构建一系列互补的铁响应生物报告，能够感测广泛的生物可利用的铁。类似地，他们计划构建P-响应生物报告分子，该生物报告分子具有区分生物可利用的DIP和DOP种类的能力。尽管磷限制是现代湖沼学的核心原则，但直到最近才在海洋学家中得到更广泛的关注。然而，低表面水平的P与世界上许多海洋有关。特别是，北大西洋西部地区和地中海一样，都是磷有限的。这项建议的一个实地组成部分是参加在马尾藻海的一次机会巡航。.就更广泛的影响而言，该项目将以特别的教育推广活动为特色。 自2004年以来，BGSU一直参与与洛兰县社区学院（LCCC）通过他们的创新大学合作伙伴关系（UP）计划的伙伴关系。UP的创建是为了向洛兰和周边县的居民提供服务，这些县是俄亥俄州的一部分？经济不景气吗？锈带？有机会获得学士学位，同时参加LCCC。通过UP，BGSU等机构提供教师和课程，通过远程学习增加LCCC的课程。 为了促进研究，LCCC学生将与BGSU教师共同顾问配对，该顾问与LCCC的同行合作，以便学生可以使用LCCC实验室设施进行他/她的项目。