Novel Biosensor Technology for the Determination of Bioavailable Iron in Aquatic Ecosystems

用于测定水生生态系统中生物可利用铁的新型生物传感器技术

• 批准号: 9911592
• 负责人: Robert McKay
• 金额: $ 16.84万
• 依托单位: Bowling Green State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9911592&HistoricalAwards=false
• 关键词: Novel; Biosensor; Technology; Determination; Bioavailable

ABSTRACTOCE-9911592Despite increasing recognition that iron distribution and availability is significant in terms of global oceanic production, the biogeochemistry of this trace metal remains poorly characterized. In particular, it particularly desirable to develop techniques for estimating what portion of iron is available for utilization by the phytoplankton. In this project, scientists at the Bowling Green State University will construct a novel, genetically-engineered cell biosensor for use in the determination of bioavailable iron in the waters of the Laurentian Great Lakes. The Cyanobacterium Synechococcus sp. PCC7942 will be used for the construction of the biosensor, and biosensor response will be evaluated in short-term Fe-limited growth-rate experiments, iron additions, and in studies to investigate the influences of temperature and light. In the field, the research team will study the biosensor performance in on-deck incubations of water collected at discrete depth intervals as well as in in-situ deployments to discrete depths. Biosensor response will be compared to other independent chemical measurements of bioavailable iron. Once the technology is developed and perfected in the Great Lakes, it will be possible to begin development of a similar tool for seawater.

摘要-9911592尽管越来越多的人认识到铁的分布和可利用性在全球海洋生产方面具有重要意义，但这种痕量金属的地球化学特征仍然很差。 特别是，它特别希望开发技术，估计铁的哪一部分是可利用的浮游植物。 在这个项目中，鲍林绿色州立大学的科学家将构建一种新型的基因工程细胞生物传感器，用于测定劳伦特五大湖沃茨中的生物可利用铁。 蓝细菌Synechococcus sp. PCC 7942将用于构建生物传感器，生物传感器响应将在短期铁限制生长速率实验、铁添加以及研究温度和光照影响的研究中进行评估。 在该领域，研究小组将研究生物传感器在甲板上培养以离散深度间隔收集的水以及在离散深度的原位部署中的性能。 将生物传感器响应与生物可利用铁的其他独立化学测量进行比较。 一旦这项技术在五大湖得到发展和完善，就有可能开始为海水开发类似的工具。