Collaborative Research: Development and Field use of Cyanobacterial Iron-Dependent Bioreporters

合作研究：蓝藻铁依赖性生物报告器的开发和现场使用

• 批准号: 0327738
• 负责人: Robert McKay
• 金额: $ 22.57万
• 依托单位: Bowling Green State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0327738&HistoricalAwards=false
• 关键词: Collaborative; Research; Development; Field; use

ABSTRACTOCE-0327738OCE-0327730Researchers at Bowling Green State University and Clarkson University will collaborate to develop a series of luminescent cyanobacterial Fe-dependent bioreporter strains capable of quantifying the bioavailability of iron in aquatic environments. This project will use the same genetic techniques and expanded analytical approaches used to develop a prototype freshwater bioreporter with the cyanobacterium Synechococcus sp. PCC 7942. By analyzing dose-response curves generated for each promoter, the investigators hope to construct a series of bioreporters with an expanded dynamic range of detection of bioavailable iron. While the information provided from this study will address a gap in our current knowledge on the availability of iron to freshwater organisms, the investigators expect that it will eventually be applicable to the development of analogous marine sensors.

鲍林格林州立大学和克拉克森大学的研究人员将合作开发一系列发光蓝藻铁依赖生物报告菌株，能够量化水环境中铁的生物有效性。该项目将使用相同的基因技术和扩展的分析方法来开发蓝藻聚球藻原型淡水生物报告程序。PCC 7942。通过分析每个启动子产生的剂量-反应曲线，研究人员希望构建一系列生物记者，扩大生物可利用铁的检测动态范围。虽然这项研究提供的信息将弥补我们目前在淡水生物铁可获得性方面的知识空白，但调查人员预计，它最终将适用于开发类似的海洋传感器。