Newly engineered ZnO nanoplatforms and their initial evaluation in ultrasensitive biomedical marker detection

新设计的 ZnO 纳米平台及其在超灵敏生物医学标记物检测中的初步评估

• 批准号: 0729541
• 负责人: Jong-in Hahm
• 金额: --
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-01 至 2010-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0729541&HistoricalAwards=false
• 关键词: Newly; engineered; ZnO; nanoplatforms; their

0729541HahmThe objective of the project is to utilize recent advances in nanomaterials to develop improved tools for the detection and quantification of medically relevant protein markers. The research integrates engineering principles of the optical enhancement effect of zinc oxide nanorods (ZnO NRs) in detecting early disease biomarkers. The intellectual merit of the proposed research lies in both fundamental and engineering aspects. The project presents a scheme where a single-step growth will produce ZnO NRs of a uniform size, shape, density, and orientation that are assembled in an array format, directly after synthesis. The project will also ascertain the exact mechanism dictating the important phenomenon of ZnO NR-mediated fluorescence enhancement. The research could then demonstrate that the easily fabricated ZnO NRs will enable high sensitivity protein detection without the need for chemical/enzymatic amplification or specialized instrumentation. These ZnO NR-based protein sensors could have superior detection capabilities than the currently available techniques. The project results could lead to the development of sensitive, low-cost assays of biomedically relevant markers of human disease even at ultratrace levels. The combined growth and assembly process proposed in this research will be applied straightforwardly and conveniently to produce ZnO nanosensor arrays that are compatible with conventional automatic sample handlers and fluorescence readers. This advantage can promote the widespread applications of these nanoplatforms in basic biology, clinical testing, and biomedical research. This versatile platform could not only allow early detection of various disease biomarkers but also facilitate detection of environmental hazards and chemical and biological threats.The proposed research does have a potential to advance discovery and understanding fluorescence detection capability of ZnO nanorods. The project includes the students at graduate and undergraduate levels in both chemical and biomedical engineering.

该项目的目标是利用纳米材料的最新进展来开发改进的工具，用于检测和量化与医学相关的蛋白质标记。这项研究结合了氧化锌纳米棒在检测早期疾病生物标志物中的光学增强效应的工程原理。这项研究的学术价值在于基础和工程两个方面。该项目提出了一种方案，其中一步生长将产生尺寸、形状、密度和取向均匀的氧化锌纳米管，这些纳米管在合成后直接组装成阵列格式。该项目还将确定决定氧化锌NR介导的荧光增强这一重要现象的确切机制。然后，这项研究可以证明，容易制造的氧化锌近红外光谱将能够实现高灵敏度的蛋白质检测，而不需要化学/酶放大或专门的仪器。这些基于氧化锌天然橡胶的蛋白质传感器可能具有比目前可用的技术更好的检测能力。该项目的结果可能导致开发敏感的、低成本的人类疾病生物医学相关标记的分析，即使在超种族水平上也是如此。本研究中提出的生长和组装相结合的工艺将直接和方便地应用于制备与传统的自动样品处理机和荧光读取器兼容的氧化锌纳米传感器阵列。这一优势可以促进这些纳米平台在基础生物学、临床测试和生物医学研究中的广泛应用。这个多功能的平台不仅可以早期检测各种疾病生物标志物，还可以方便地检测环境危害和化学和生物威胁。拟议的研究确实有可能促进发现和了解纳米棒的荧光检测能力。该项目包括化学和生物医学工程专业的研究生和本科生。