EFRI-CBE: Emerging Frontiers in 3-D Breast Cancer Tissue Test Systems

项目名称：K.J. Burg机构：克莱姆森大学提案号：0736007 EFRI-CBE: 3d乳腺癌组织检测系统的新兴前沿摘要该项目的愿景是开发和展示一种新的，综合的方法来研究乳腺癌组织中癌细胞的复杂行为。该方法的核心目标是建立一个体外工程组织结构，再现正常细胞和癌细胞的体内行为，并允许操纵环境参数来建立因果模型。具体地说，拟议工作的终点将是描述乳腺癌细胞和生物分子行为的数学关系，作为氧水平和组织刚度的相互作用，从而增加对疾病机制的理解。提出的方法将需要开发新的实验和分析工具来制造分层结构的组织，并在此框架内评估细胞行为。开发的关键工具包括3D组织制造系统，将癌细胞迁移和增殖与组织氧和刚度水平联系起来的现实数学模型，以及一种新型纳米颗粒氧传感器。该项目将导致3D制造系统和统计方法的发展，能够以可重复的方式构建组织。也就是说，制造的组织可以用作个性化诊断，用于优化现有治疗方法、开发新药和开发新疫苗。动物科学、生物学、化学、工程学、临床医学和统计学之间的思想和方法的交叉授粉将从这项拟议的工作中产生，这为研究人员提供了一个独特的培训机会。参与拟议的多学科团队将为个人调查员提供对自己领域的新见解。参与该计划的学生将沉浸在一个技术多样化的环境中，包括科学家、工程师和临床医生，并将磨练许多对2020年工程师或科学家至关重要的技能，包括沟通、领导和团队合作。通过精心设计的教育大纲和社区外展计划，不同群体的学生（k -研究生）和教育工作者（K-12）将参与其中。

PI name: K.J. Burg Institution: Clemson University Proposal Number: 0736007 EFRI-CBE: Emerging Frontiers in 3-D Breast Cancer Tissue Test Systems AbstractThe vision for this project is to develop and demonstrate a new, integrative means of studying the complex behavior of cancer cells in breast tissue. The approach centers on the goal of building an in vitro engineered tissue structure that reproduces the in vivo behavior of the normal and cancerous cells and allows manipulation of environmental parameters to build cause and affect models. Specifically, the end-point of the proposed work will be mathematical relationships describing breast cancer cellular and biomolecular behavior as interactive functions of oxygen level and tissue stiffness, and thus an increased understanding of disease mechanisms. The proposed approach will require development of new experimental and analytical tools to fabricate tissues of hierarchical structure and to assess cell behavior within this framework. The key tools to be developed include a 3D tissue fabrication system, realistic mathematical models to relate cancer cell migration and proliferation with tissue oxygen and stiffness levels, and a novel nanoparticle oxygen sensor.This project will result in the development of a 3D fabrication system and statistical methodology capable of building tissue in repeatable manner. That is, the fabricated tissue may be used as a personalized diagnostic with which to optimize existing therapeutics, develop new drugs, and develop new vaccines. The cross-pollination of ideas and methods between animal science, biology, chemistry, engineering, clinical medicine, and statistics that will result from this proposed work represents a unique training opportunity for the Investigators. Participation on the proposed multi-disciplinary team will provide individual investigators new insights into their own field. Students involved in the proposed program will be immersed in a technically diverse environment including scientists, engineers, and clinicians, and will hone many skills crucial to the 2020 engineer or scientist, including communication, leadership, and teamwork. Through a carefully crafted educational rubric as well as community outreach program, a diverse population of students (K-postgraduate) and educators (K-12) will be engaged.