PECASE: Analysis of Novel Composite Tissue Engineered Systems

[00:93805]研究和开发工程组织具有潜在的广泛的医学应用，包括乳房肿瘤切除重建、肝脏修复、软骨置换或其他疾病。组织工程包括从病人身上提取少量健康细胞，在实验室环境中培养成大量细胞，将它们与一种可吸收的（类似于可生物降解的）材料结合起来，这种材料已经被雕刻成受伤部位的解剖形状，允许细胞发育，将细胞-材料系统植入病人体内，然后让细胞生长并获得原始的解剖形状。这些物质逐渐分解，被身体“吸收”，从而被排出体外。这项计划为期5年的工作重点是为接受乳房切除术或乳房肿瘤切除术的癌症患者提供乳腺组织工程的基础科学。我们将努力发展一个组织工程项目，包括新的注射材料的研究和开发以及研究生和本科生的培训。它还将通过开发K-12教育推广计划来提高公众对生物工程和批判性思维的认识，该计划是由克莱姆森大学生物工程系和教育部共同努力开发的。提出的研究任务是提出和证明一种新的材料方法来组织工程。据推测，通过提供两相、复合、可注射的材料，细胞生长和组织发育可能在临床可行的解决方案中得到加强。申请人以前的研究已经证明了这些系统的一般功效，但没有包括对组成部分的仔细评估。选择不同比例的工艺材料将被处理，研究将使用全面的机械和化学测试来评估系统。基础研究概念的成功依赖于一个精心策划的研究计划，以及对参与其中的科学家和未来科学家的教育。从本工作中获得的科学技术将被转移到其他有类似要求的临床应用中。

0093805Karen BurgResearch and development of engineered tissue has potential widespread medical application, including lumpectomy reconstruction, liver repair, cartilage replacement, or other conditions. Tissue engineering involves taking a small number of healthy cells from a patient, growing the cells into large numbers in a laboratory environment, combining them with an absorbable (similar to biodegradable) material that has been sculpted into the anatomical shape of the injured part, allowing the cells to develop, implanting the cell-material system into the patient, and then allowing the cells to grow and acquire the original anatomical shape. The material gradually breaks down, is "absorbed" by the body, and is thus removed.The proposed 5-year work is a focus on the basic science of breast tissue engineering for cancer patients who have undergone a mastectomy or lumpectomy. The effort will be to develop a program in tissue engineering, encompassing the research and development of new injectable materials and training of graduate and undergraduate students. It also will promote public awareness of bioengineering and critical thinking through the development of a K-12 educational outreach program, a program developed through the combined efforts of the Department of Bioengineering and the Department of Education at Clemson University. The mission of the proposed research is to present and justify a new materials approach to tissue engineering. It is hypothesized that by providing a two-phase, composite, injectable material, cell growth and tissue development may be enhanced within a clinically viable solution. Previous studies by the applicant have demonstrated general efficacy of such systems, but have not encompassed a careful assessment of the components. A selection of crafted materials of varying proportions will be addressed and the research will be conducted using a comprehensive battery of mechanical and chemical tests to assess the systems. The success of the basic research concept relies on a well-orchestrated research plan, as well as on education of the involved scientists and future scientists. The science and technology derived from this work will be transferred to other clinical applications presenting similar requirements.