Identification and characterisation of novel components in glucosinolate biosynthesis and regulation

R2 R3-MYB转录因子家族亚组12的6个成员（MYB 51、MYB 34、MYB 122、MYB 28、MYB 29和MYB 76）已被证明参与拟南芥中芥子油苷生物合成途径的调节。在这个项目中，我们的目标是获得新的见解，这些转录因子的调控，并解开未知的上游组件和相互作用的伙伴MYBs。为此，（i）转录控制亚组12 MYB因子的上游调控，如SLIM 1，Dof1.1和IQD 1将被研究，（ii）他们的翻译后调控通过磷酸化/去磷酸化（MPK 3，MPK 6，MKP 1和PTP 1）将被分析，（iii）蛋白质相互作用与这些MYB因子在酵母双杂交和在植物中将被详细研究。此外，我们的目标是研究新的转运蛋白参与细胞内和细胞间的硫代葡萄糖苷（GS）或GS生物合成前体的运输。目的是（iv）表征3 '-磷酸腺苷5'-磷酸硫酸盐（PAPS）的新型叶绿体cPAPST 1转运蛋白，（v）阐明cPAPST 1、cPAPST 2和APS激酶的作用，APK 1、APK 2、APK 3和APK 4在PAPS和PAP分配中的作用（腺苷3 '，5'-二磷酸）之间的叶绿体和胞质溶胶，和（vi）确定和验证新的长距离转运脂肪族和吲哚GS。

The six members of subgroup 12 of the R2R3-MYB transcription factor family (MYB51, MYB34, MYB122, MYB28, MYB29 and MYB76) have been shown to be involved in the regulation of the glucosinolate biosynthetic pathway in Arabidopsis thaliana. In this project we aim to get new insights about the regulation of these transcription factors and to unravel the unknown upstream components and interacting partners of MYBs. To this end, (i) transcriptional control of subgroup 12 MYB factors by upstream regulators like SLIM1, Dof1.1 and IQD1 will be studied, (ii) their posttranslational regulation via phosphorylation/dephosphorylation (MPK3, MPK 6, MKP1 and PTP1) will be analysed, (iii) proteins interacting with these MYB factors both in yeast two hybrid and in planta will be studied in detail. In addition, we aim to study novel transporters involved in the intra- and inter-cellular transport of glucosinolates (GS) or GS biosynthetic precursors. It is aimed to (iv) characterize the novel chloroplastidic cPAPST1 transporter of 3'-phosphoadenosine 5'- phosphosulfate (PAPS), (v) elucidate the role of cPAPST1, cPAPST2 and of APS kinases, (APK1, APK2, APK3 and APK4) in the partitioning of PAPS and PAP (adenosine 3‘,5‘-bisphosphate) between chloroplasts and the cytosol, and, (vi) identify and characterise novel long distance transporters of aliphatic and indolic GS.