Dynamics of regulatory protein complexes in a changing environment: using the example of plant glucosinolate biosynthesis

环境变化中调节蛋白复合物的动态：以植物芥子油苷生物合成为例

• 批准号: 322540466
• 负责人: Privatdozentin Dr. Tamara Gigolashvili
• 金额: --
• 依托单位: Arbeitsgruppe Gigolashvili (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/322540466?language=en
• 关键词: Dynamics; regulatory; protein; complexes; changing

Transcriptional control involves the formation of complexes between a discrete number of transcription factors (TFs) and regulatory proteins and their subsequent interactions with gene regulatory DNA regions to convey signals to RNA Polymerase-II. These signals, in turn, instruct RNA Polymerase whether to transcribe genes and to what extent. This proposal aims to understand how the information encoded by DNA is transformed into specific cellular responses and to study the mechanisms that underlie the formation of TF complexes in response to specific intra- or extracellular signals. While studying the assembly and disassembly of regulatory complexes in response to specific signals, we will address the interactions between complex components and reveal mechanisms that explain the ability of TFs to activate specific cellular responses by regulating a selected set of genes. In contrast to prokaryotes, the regulation of gene transcription in eukaryotes is controlled not only at the level of transcription, but also epigenetically, post-transcriptionally, translationally and post-translationally. However, with the exception of hormone receptors in eukaryotes and a few primary metabolites in yeast, little is known concerning how small molecules can modulate TF activity or TF complex assembly. In this proposal, we will address how the activity of proteins belonging to the MYB-bHLH regulatory complexes is modulated in response to signalling molecules, using the example of particular class of plant secondary metabolites, namely, glucosinolates (GSLs). Knowledge gained in this project is expected to extend knowledge on the dynamics of regulatory protein-ligand assembly and disassembly in changing extracellular environments, using the example of the GSL biosynthesis pathway. The results will promote the understanding of metabolite signalling in GSL biosynthesis and will contribute to the understanding of how the activity of regulatory proteins can be generally controlled by the metabolic products. The project will generate insight into the mechanisms by which TFs can activate specific cellular responses in a given environment.

转录控制涉及在离散数量的转录因子（TF）和调控蛋白之间形成复合物，以及它们随后与基因调控DNA区域相互作用以将信号传递到RNA聚合酶-II。这些信号反过来又指示RNA聚合酶是否转录基因以及转录到什么程度。该提案旨在了解DNA编码的信息如何转化为特定的细胞反应，并研究TF复合物形成的机制，以响应特定的细胞内或细胞外信号。在研究调节复合物响应特定信号的组装和拆卸时，我们将讨论复杂组分之间的相互作用，并揭示解释TF通过调节选定的一组基因来激活特定细胞反应的能力的机制。与原核生物相比，真核生物中的基因转录调控不仅在转录水平上受到控制，而且在表观遗传学上、转录后、转录后和转录后都受到控制。然而，除了真核生物中的激素受体和酵母中的一些初级代谢产物外，关于小分子如何调节TF活性或TF复合物组装知之甚少。在这项提案中，我们将解决如何调节的MYB-bHLH调节复合物的蛋白质的活性响应于信号分子，使用特定类别的植物次生代谢产物，即芥子油苷（GSL）的例子。在这个项目中获得的知识，预计将扩展知识的动态变化的细胞外环境中的调节蛋白-配体组装和拆卸，使用的GSL生物合成途径的例子。这些结果将促进对GSL生物合成中代谢物信号传导的理解，并将有助于理解调节蛋白的活性如何通常由代谢产物控制。该项目将深入了解TF在给定环境中激活特定细胞反应的机制。