SBIR Phase I: A Robust Optical Biosensor for Cardiac Diagnostics

这项小型企业创新研究(SBIR)第一阶段研究项目旨在开发一种基于表面等离子体共振(SPR)和公共路径干涉检测(CPI)的新型检测系统，以检测具有生物相关性的结合事件。研究人员将使用自组装单分子层(SAM)化学，即由聚合物组成的三维结合表面来结合感兴趣的抗体，这些抗体将反过来从血液或其他生物液中捕获感兴趣的抗原。结合事件的当前检测依赖于标记分子之一，并随后监测标记的实体与感兴趣的目标的相互作用。因此，与这种测量相关的成本和工作量很高，如果消除了对标签的需要，成本和工作量可以显著降低。目前的提案解决了这一关键问题，并可能提供一种在医疗和科学应用中有用的解决方案。一种基于SPR和CPI的检测有可能成功地实现这一重要问题。

This Small Business Innovation Research (SBIR) Phase I research project aims to develop a novel detection system based on surface plasmon resonance (SPR) and common path interferometric detection (CPI), to detect binding events of biological relevance. The investigators will use self assembled monolayer (SAM) chemistry, or a three dimensional binding surface comprised of polymers to bind the antibodies of interest that will in turn, capture antigens of interest from the blood or other biological fluids. Current detection of binding events relies on labeling one of the molecules and subsequently monitoring the interaction of the labeled entity with the target of interest. As such, the cost and effort associated with such measurements are high and could be significantly lowered if the need for labeling is obviated. The current proposal addresses this crucial problem and may provide a solution that would be of use in medical and scientific applications. An assay based on SPR and CPI has the potential to successfully achieve this important issue.