Dissecting the ROS Signaling Network of Cells

剖析细胞的 ROS 信号网络

• 批准号: 0743954
• 负责人: Gary Blomquist
• 金额: $ 48万
• 依托单位: Board of Regents, NSHE, obo University of Nevada, Reno
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-15 至 2011-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0743954&HistoricalAwards=false
• 关键词: Dissecting; ROS; Signaling; Network; Cells

Reactive oxygen species (ROS) control many different processes in cells including cell death, stress responses, aging and cancer. Because the potential risk of oxidative stress is common to all aerobic organisms, elucidating the ROS-signaling network of plant cells would have a significant impact in medicine and agriculture. The long-term goal of the project is to dissect the ROS signaling network of cells and determine how ROS signals are sensed and transduced in plant cells. The experimental approach employed in the program will be to use a combination of genetic, molecular, biochemical and bioinformatic research tools to identify, clone and characterize different genes involved in ROS signaling in cells. The identification of genes involved in ROS sensing, the pathway for ROS signaling, and the collection of ROS-mutants, generated during the course of the proposed research, would serve as a valuable resource for the entire scientific community. The information generated from the project will enhance overall understanding of plant biology, and help determine to what extent and in what manner ROS are involved in different biological process. Broader impacts. To increase interest and understanding of science in young people, high school students and undergraduates, including minorities, will be recruited into the project and trained in ROS biology, molecular biology, and bioinformatics. This project will provide training to 2 graduate students, 8 undergraduates and 8 high school students. In addition, four 1 week summer workshops, 25 high school students each, are planned for the duration of the grant. The proposed project will provide interdisciplinary training that has become imperative for success in plant sciences. To enhance the awareness of high school students, teachers and undergraduates, two teaching-oriented websites targeted to high school students and undergraduates, and an online presentation targeted to high school teachers will be developed to share knowledge and enhance awareness to ROS metabolism in plants, mammals and other organisms.

活性氧 (ROS) 控制细胞中的许多不同过程，包括细胞死亡、应激反应、衰老和癌症。由于氧化应激的潜在风险对于所有需氧生物体来说都是常见的，因此阐明植物细胞的 ROS 信号网络将对医学和农业产生重大影响。该项目的长期目标是剖析细胞的ROS信号网络，并确定ROS信号在植物细胞中如何感知和转导。该项目采用的实验方法将结合遗传、分子、生物化学和生物信息学研究工具来识别、克隆和表征细胞中参与 ROS 信号传导的不同基因。在拟议的研究过程中产生的与 ROS 传感相关的基因、ROS 信号通路以及 ROS 突变体的收集的鉴定，将成为整个科学界的宝贵资源。该项目产生的信息将增强对植物生物学的整体理解，并有助于确定ROS在多大程度上以及以何种方式参与不同的生物过程。更广泛的影响。为了提高年轻人对科学的兴趣和理解，该项目将招募高中生和本科生（包括少数族裔），并接受 ROS 生物学、分子生物学和生物信息学方面的培训。该项目将培训研究生2名、本科生8名、高中生8名。此外，在赠款期间还计划举办四次为期 1 周的夏季研讨会，每次有 25 名高中生参加。拟议的项目将提供跨学科培训，这对于植物科学的成功至关重要。为了提高高中生、教师和本科生的认识，将开发两个针对高中生和本科生的教学网站和一个针对高中教师的在线演示，以分享知识并提高对植物、哺乳动物和其他生物体中活性氧代谢的认识。