Positional Signaling in Arabidopsis Root Epidermis Development

姓名（N）：John Schiefelbein提案编号：拟南芥根表皮发育中的位置信号正常发育的特征是在适当的位置和模式形成细胞、组织和器官。 发育生物学领域的一个主要挑战是了解不同的细胞类型如何以适当的模式出现。 本计画将以拟南芥根表皮发育为简单模式，探讨此问题。 在先前的工作中，发现一种新的受体（命名为SCRAMBLED（SCM））在使发育中的表皮细胞能够解释其相对于底层根细胞的位置方面发挥关键作用。提出了一个工作模型，即SCM受体的影响网络的转录调控在一个位置依赖性的方式产生观察到的细胞类型的模式在根表皮。在本研究项目中，将通过使用分子和遗传学方法分析SCM受体的积累和特异性功能来测试该模型。 此外，可能以SCM相关方式起作用的新基因将在遗传筛选和基于差异表达的微阵列筛选中鉴定。研究结果有望为多细胞生物发育过程中细胞规格和模式形成的分子调控提供令人兴奋的新见解。 此外，该项目将在若干领域产生更广泛的影响。 它将为本科生、研究生和博士后提供现代生物学研究的教育机会。 该项目还将通过一个网站（http：//www.mcdb.lsa.umich.edu/labs/jumefel/index.html）向拟南芥研究界和公众提供信息和教育材料。此外，研究结果将被纳入正式的教育活动，包括一个新的本科生发育生物学课程和一个跨学科的培训计划，为本科生在生物和数学科学，沉浸在实验室和理论研究的学生。

Name: John SchiefelbeinProposal Number: IOS-0744599Title: Positional Signaling in Arabidopsis Root Epidermis DevelopmentNormal development is characterized by the formation of cells, tissues, and organs in appropriate places and patterns. A major challenge in the field of developmental biology is to understand how distinct cell types arise in appropriate patterns. This project will address this issue by using root epidermis development in Arabidopsis as a simple model. In prior work, a new receptor (named SCRAMBLED (SCM)) was found to play a critical role in enabling developing epidermal cells to interpret their position relative to the underlying root cells. A working model was proposed whereby the SCM receptor influences a network of transcriptional regulators in a position-dependent manner to generate the observed cell-type pattern in the root epidermis. In this research project, this model will be tested by analyzing the accumulation and the specific function of the SCM receptor using molecular and genetic methods. In addition, new genes that are likely to act in a SCM-related manner will be identified in a genetic screen and in a differential expression-based microarray screen. The research results are expected to provide exciting new insights into the molecular regulation of cell specification and pattern formation during the development of multicellular organisms. Further, this project will have a broader impact in several areas. It will provide educational opportunities for undergraduates, graduate students, and postdoctoral students in modern biological research. The project will also provide information and educational material to the Arabidopsis research community and the general public, via a website (http://www.mcdb.lsa.umich.edu/labs/schiefel/index.html). Furthermore, the results will be incorporated into formal educational activities, including a new undergraduate developmental biology course and an interdisciplinary training program for undergraduates in biological and mathematical sciences, which immerses students in laboratory and theoretical research.