Positional Signaling in Arabidopsis Root Epidermis Development

名称：John Schiefelbein建议编号：IOS-0744599标题：拟南芥根表皮发育中的位置信号正常发育的特征是在适当的位置和模式形成细胞、组织和器官。发育生物学领域的一个主要挑战是了解不同类型的细胞如何以适当的模式出现。这个项目将通过使用拟南芥的根表皮发育作为一个简单的模型来解决这个问题。在以前的工作中，发现了一种新的受体(名为scrbled(SCM))，它在使发育中的表皮细胞能够解释它们相对于根细胞的位置方面发挥了关键作用。提出了一个工作模型，通过该模型，SCM受体以位置依赖的方式影响转录调节网络，以在根表皮中产生观察到的细胞类型模式。在本研究项目中，将通过使用分子和遗传学方法分析SCM受体的积累和特定功能来验证这一模型。此外，可能以SCM相关方式起作用的新基因将在基因筛查和基于差异表达的微阵列筛查中被识别。这些研究结果有望为多细胞生物体发育过程中细胞规格和图案形成的分子调控提供令人兴奋的新见解。此外，该项目将在几个领域产生更广泛的影响。它将为本科生、研究生和博士后提供现代生物学研究的教育机会。该项目还将通过网站(http://www.mcdb.lsa.umich.edu/labs/schiefel/index.html).向拟南芥研究界和公众提供信息和教育材料此外，这些成果将被纳入正式的教育活动，包括一门新的本科发育生物学课程，以及一项针对生物和数学科学本科生的跨学科培训计划，让学生沉浸在实验室和理论研究中。

Name: John SchiefelbeinProposal Number: IOS-0744599Title: Positional Signaling in Arabidopsis Root Epidermis DevelopmentNormal development is characterized by the formation of cells, tissues, and organs in appropriate places and patterns. A major challenge in the field of developmental biology is to understand how distinct cell types arise in appropriate patterns. This project will address this issue by using root epidermis development in Arabidopsis as a simple model. In prior work, a new receptor (named SCRAMBLED (SCM)) was found to play a critical role in enabling developing epidermal cells to interpret their position relative to the underlying root cells. A working model was proposed whereby the SCM receptor influences a network of transcriptional regulators in a position-dependent manner to generate the observed cell-type pattern in the root epidermis. In this research project, this model will be tested by analyzing the accumulation and the specific function of the SCM receptor using molecular and genetic methods. In addition, new genes that are likely to act in a SCM-related manner will be identified in a genetic screen and in a differential expression-based microarray screen. The research results are expected to provide exciting new insights into the molecular regulation of cell specification and pattern formation during the development of multicellular organisms. Further, this project will have a broader impact in several areas. It will provide educational opportunities for undergraduates, graduate students, and postdoctoral students in modern biological research. The project will also provide information and educational material to the Arabidopsis research community and the general public, via a website (http://www.mcdb.lsa.umich.edu/labs/schiefel/index.html). Furthermore, the results will be incorporated into formal educational activities, including a new undergraduate developmental biology course and an interdisciplinary training program for undergraduates in biological and mathematical sciences, which immerses students in laboratory and theoretical research.