Functions of Conserved Oomycete Effector Proteins

保守卵菌效应蛋白的功能

基本信息

项目摘要

Oomycete plant pathogens cause extremely destructive diseases on crops and in natural ecosystems. Little is known about the molecular weapons that enable oomycetes to be such successful pathogens. Analyses of four recently completed oomycete genomes suggest that oomycetes export dozens or hundreds of their own 'effector' proteins to the interior of plant cells. These pathogen effectors are expected to sabotage plant immune responses and trigger other effects that benefit the pathogen. However, almost nothing is known about the molecular functions of oomycete effectors. This project will investigate the functions of six pairs of effectors that are conserved between two distantly related oomycetes: the Arabidopsis downy mildew pathogen Hyaloperonospora parasitica and the soybean root rot pathogen Phytophthora sojae. It is expected that a focus on conserved effectors will reveal insights that are applicable to the majority of oomycete pathogens. The investigators will perform a series of molecular genetic experiments to determine whether these effectors are capable of suppressing plant immunity or altering plant cell structure or physiology, and whether the pathogen's ability to cause disease is affected when these genes are silenced or overexpressed. Finally, one pair of conserved proteins will be selected for experiments to identify their exact targets inside plant cells. These experiments are expected to reveal important, broadly conserved molecular strategies that underpin oomycetes' ability to cause disease, which will in turn open new avenues toward more sophisticated disease control strategies. The project will provide multidisciplinary training for graduate students and undergraduates. Some aspects of the project will be collaboratively integrated with an outreach program at Virginia Tech that uses Arabidopsis reverse genetics in high school science classrooms.
卵菌类植物病原体对农作物和自然生态系统造成极具破坏性的疾病。人们对卵菌成为如此成功的病原体的分子武器知之甚少。对最近完成的四个卵菌基因组的分析表明,卵菌向植物细胞内部输出数十或数百种自己的“效应器”蛋白质。这些病原体效应物预计会破坏植物的免疫反应,并触发有利于病原体的其他效应。然而,对卵菌效应器的分子功能几乎一无所知。本项目将研究在两个远缘卵菌之间保守的6对效应子的功能:拟南芥霜霉病病原菌Hyaloponospora parasitica和大豆根腐病病原菌Phytophthora sojae。预计对保守效应因子的关注将揭示适用于大多数卵菌病原体的见解。研究人员将进行一系列分子遗传学实验,以确定这些效应器是否能够抑制植物免疫或改变植物细胞结构或生理,以及当这些基因沉默或过度表达时,病原体的致病能力是否受到影响。最后,将选择一对保守的蛋白质进行实验,以确定它们在植物细胞内的确切靶标。这些实验有望揭示重要的、广泛保守的分子策略,这些策略支持卵菌致病的能力,这反过来将为更复杂的疾病控制策略开辟新的途径。该项目将为研究生和本科生提供多学科培训。该项目的某些方面将与弗吉尼亚理工大学的一个推广计划合作整合,该计划在高中科学课堂上使用拟南芥反向遗传学。

项目成果

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John McDowell其他文献

Kant’s Theoretical Philosophy: The ‘Analytic’ Tradition
康德的理论哲学:“分析”传统
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    James R. O’Shea;S. Baiasu;eds Mark Timmons;Wilfrid S. Sellars;Gareth Evans;Hilary Putnam;Donald Davidson;Michael Dummett;John McDowell;Rae Langton;R. Brandom
  • 通讯作者:
    R. Brandom
Physicalism and primitive denotation: Field on Tarski
  • DOI:
    10.1007/bf00160891
  • 发表时间:
    2004-06-26
  • 期刊:
  • 影响因子:
    0.900
  • 作者:
    John McDowell
  • 通讯作者:
    John McDowell
European Journal of Pragmatism and American Philosophy, XII-1 | 2020
欧洲实用主义与美国哲学杂志,XII-1 |
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Macarthur;John McDowell
  • 通讯作者:
    John McDowell
De Re Senses

John McDowell的其他文献

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{{ truncateString('John McDowell', 18)}}的其他基金

EDGE FGT: RNAi-based tools to unlock functional genomics of obligate oomycete plant pathogens
EDGE FGT:基于 RNAi 的工具,用于解锁专性卵菌植物病原体的功能基因组学
  • 批准号:
    2319757
  • 财政年份:
    2023
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Intergovernmental Personnel Award
政府间人才奖
  • 批准号:
    2200404
  • 财政年份:
    2021
  • 资助金额:
    $ 48万
  • 项目类别:
    Intergovernmental Personnel Award
Technician Training in CRISPR-based Gene Editing
基于 CRISPR 的基因编辑技术人员培训
  • 批准号:
    2000696
  • 财政年份:
    2020
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Technician Training in Gene Editing
基因编辑技术人员培训
  • 批准号:
    1700660
  • 财政年份:
    2017
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Dissertation Research: Social Networks and Adaptive Strategies of Inupiaq Households
论文研究:因纽特人家庭的社交网络和适应策略
  • 批准号:
    0331825
  • 财政年份:
    2003
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant

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HMA 结构域蛋白作为利用胞间连丝的病原体的保守靶标
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Investigation of conserved proteins that regulate transmission of the malaria parasite and Toxoplasma gondii.
研究调节疟疾寄生虫和弓形虫传播的保守蛋白。
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研究-PGR:原子序数:识别驱动植物元素积累的保守基因
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Collaborative Research: How to manipulate a plant? Testing for conserved effectors and plant responses in gall induction and growth using a multi-species comparative approach.
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RUI:使用新型激动剂和比较鱼类模型确定脑中表达的超级保守受体 (SREB) G 蛋白偶联受体家族的生殖作用
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    2307614
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阐明植物免疫背后的保守基因调控网络
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