CAREER: Histone-Mimetic Gold Nanoparticles as Self-Activating and Tailorable Gene Delivery Scaffolds

职业：组蛋白模拟金纳米颗粒作为自激活和可定制的基因传递支架

• 批准号: 0746458
• 负责人: Millicent Sullivan
• 金额: $ 48.98万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0746458&HistoricalAwards=false
• 关键词: CAREER; Histone; Mimetic; Gold; Nanoparticles

0746458SullivanThis Career award to University of Delaware by the Biomaterials program in the Division of Materials Research is to study a novel, self-activating gene delivery platform by combining self-assembled monolayer-functionalized with nanogold particles with attached biomimetic and nucleus-specific unpackaging peptides. With this award, Dr. Millicent Sullivan will synthesize and characterize histone-mimetic gold nanoparticles (HMGNs) modified with trimethylated histone tails (H3K4Me3). Self-assembly of these nanoparticles with plasmid DNA will be the other part of this project. The ultimate potential goal of this project is to create HMGNs containing combinations of histone tails that optimize nucleus-specific transcriptional activation of HMGN/DNA nanocomplexes. This project, if successful, could develop an integrated gene therapy scaffold and a generic gene/drug delivery system targeted to the nucleus of the cell.One of the objectives of this project is to integrate research activities with teaching, training and education of graduate and undergraduate students at University of Delaware. The outreach component of the project involves the participation of high school students and their teachers. Local high school teachers will be selected and trained at the researcher?s laboratory as teaching interns in bioengineering course module, and these teachers in turn will provide training and internship to high school students. In addition, these high school students and teachers will be co-mentored by undergraduate and graduate students.

0746458 Sullivan这个由材料研究部生物材料计划授予特拉华大学的职业奖项是研究一种新型的、自我激活的基因传递平台，它将带有纳米金颗粒的自组装单层功能化与附着的仿生和核特定的去包装多肽结合在一起。获得该奖项后，Millicent Sullivan博士将合成并表征三甲基化组蛋白尾巴(H3K4me3)修饰的组蛋白模拟金纳米颗粒(HMGNs)。这些纳米粒子与质粒DNA的自组装将是该项目的另一个部分。该项目的最终潜在目标是创建包含组蛋白尾巴的HMGNs，以优化HMGN/DNA纳米复合体的核特异性转录激活。该项目如果成功，可能会开发出一种集成的基因治疗支架和针对细胞核的非专利基因/药物输送系统。该项目的目标之一是将研究活动与特拉华大学研究生和本科生的教学、培训和教育结合起来。该项目的外展部分涉及高中生及其老师的参与。当地的高中教师将在S研究员实验室作为生物工程课程模块的教学实习生进行培训，这些教师将依次为高中生提供培训和实习。此外，这些高中生和老师将由本科生和研究生共同指导。