CAREER: Understanding the Role of Cell Surface Markers in Microfluidic Cell Separation - An Integrated Research and Education Program

职业：了解细胞表面标记在微流控细胞分离中的作用 - 一项综合研究和教育计划

• 批准号: 0747166
• 负责人: Shashi Murthy
• 金额: $ 40万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0747166&HistoricalAwards=false
• 关键词: CAREER; Understanding; Role; Cell; Surface

CBET-0747166MurthyThis NSF award by the Chemical and Biological Separations program supports work by Professor Shashi Murthy to understand molecular-level phenomena at the surfaces of cells during separation processes in microfluidic devices. Microfluidic cell separation is one of many microscale approaches being investigated for the manipulation of cells for scientific, diagnostic, or therapeutic applications. The general principle behind the design of microfluidic cell separation systems is to selectively isolate one or more cell types from a heterogeneous suspension of several cell types. The development of microfluidic cell manipulation systems has, to date, followed an application- and technology-driven approach where knowledge of molecular-level phenomena is lacking. This proposal aims to address the existing need to understand molecular-level phenomena within the framework of an integrated research and education program. To accomplish this objective, experiments will be carried out to determine of receptor expression is increased or decreased under controlled conditions of cell flow through ligand-coated microchannels of progressively increasing geometric complexity. Based on the observed receptor response, a "fingerprint" of selected cell-ligand will be created and utilized to identify unknown cell types in heterogeneous suspensions. The Intellectual Merit of this project lies in providing a new method of identifying cells that does not rely on a priori knowledge of cell type, surface receptor type, or fluorescence staining. This method will be valuable in identifying as-yet unknown and rare stem and progenitor cell populations that are resident in certain types of tissue, such as cardiac, skin, gastrointestinal or hepatic tissue. Such identification will have significant implications in stem cell-based tissue engineering, where the ability of stem and progenitor cells to differentiate into functional cells is crucial. The Broader Impacts of this project emerge from an educational component that will utilize the PI's existing relationships with two predominantly-minority high schools to promote bioengineering by giving presentations at the schools and taking students on field trips to laboratories at Northeastern University, Massachusetts General Hospital, and Massachusetts Eye and Ear Infirmary; engages high school teachers and students, as well as undergraduates, in experimental research.

CBET-0747166 Murthy化学和生物分离项目的NSF奖项支持Shashi Murthy教授在微流体设备分离过程中了解细胞表面分子水平现象的工作。 微流控细胞分离是正在研究的用于科学、诊断或治疗应用的细胞操纵的许多微尺度方法之一。 微流体细胞分离系统设计背后的一般原理是从几种细胞类型的异质悬浮液中选择性地分离一种或多种细胞类型。 迄今为止，微流控细胞操作系统的发展遵循应用和技术驱动的方法，其中缺乏分子水平现象的知识。 该提案旨在解决现有的需要，以了解分子水平的现象在一个综合的研究和教育计划的框架内。 为了实现这一目的，将进行实验以确定在细胞流过几何复杂性逐渐增加的配体涂覆的微通道的受控条件下受体表达的增加或减少。 基于观察到的受体反应，将创建所选细胞-配体的“指纹”并用于鉴定异质悬浮液中的未知细胞类型。 该项目的智力价值在于提供一种新的识别细胞的方法，该方法不依赖于细胞类型、表面受体类型或荧光染色的先验知识。 该方法在鉴定存在于某些类型的组织（例如心脏、皮肤、胃肠道或肝组织）中的未知和罕见的干细胞和祖细胞群方面将是有价值的。 这种鉴定将在基于干细胞的组织工程中具有重要意义，其中干细胞和祖细胞分化成功能细胞的能力是至关重要的。 该项目的更广泛影响来自一个教育组成部分，该组成部分将利用PI与两所主要少数民族高中的现有关系，通过在学校发表演讲和带领学生实地考察东北大学、马萨诸塞州总医院和马萨诸塞州眼耳医院的实验室来促进生物工程;让高中教师和学生以及本科生参与实验研究。