SGER: Gold Standard Set for Functional Annotation of Microbial Hypothetical Proteins

这一小笔探索性研究拨款(SGER)将创建一个新注释的多样化基因的大型黄金标准参考集，这些基因以前被指定为假想的(具有未知功能)。这一套将作为验证和比较分析未定性基因功能注释的预期方法的基线。这个参考集的目的是克服微生物学和现代生物学的主要瓶颈之一，特别是微生物基因组测序。假想(未定性)基因约占所有预测基因的三分之一，而且随着每个新的基因组测序项目，它们的数量正在迅速扩大。几乎每天都在开发新的基因注释计算方法，以解决目前每个基因组中70%或更少的已知功能被分配的障碍。这项研究将产生200个先前假设的基因的参考集，并具有高质量的功能注释。这套资料将成为开发和验证新的和现有的基因组功能注释的新方法的宝贵资源。它还将作为现有注释方法和数据库的基准。

This Small Grant for Exploratory Research (SGER) will create a large gold standard reference set of newly annotated diverse genes that were previously designated as hypothetical (having unknown function). This set will serve as a baseline for validation and comparative analysis of prospective approaches to functional annotation of uncharacterized genes. This reference set is aimed at overcoming one of the major bottlenecks of microbiology and modern biology in general, and microbial genome sequencing in particular. Hypothetical (uncharacterized) genes constitute approximately one third of all predicted genes and their number is expanding rapidly with each new genome sequencing project. New computational approaches to gene annotation are being developed almost daily to address the hurdle of currently having 70% or less of each genome assigned with known function. This research will result in a reference set of 200 previously hypothetical genes with high quality functional annotation. This set will serve as a valuable resource for the development and validation of new methods for the function annotation of new and existing genomes. It will also serve as a benchmark for existing annotation methods and databases.